FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290
Sequences flagged as poor quality0
Sequence length150
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC224968.35866261398176No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1444.376899696048632No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGG541.6413373860182372No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC421.276595744680851No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG341.033434650455927No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.6990881458966566No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT160.48632218844984804No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.4559270516717325No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG120.364741641337386No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGC120.364741641337386No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG120.364741641337386No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG110.3343465045592705No Hit
GTCATTGTCACTGGTCAGCCCCAGCACCTTCTGCTGCGTCTCCACGTTGC110.3343465045592705No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG90.2735562310030395No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC90.2735562310030395No Hit
GTCATTTTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC90.2735562310030395No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT90.2735562310030395No Hit
GTCATTATCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC80.24316109422492402No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT80.24316109422492402No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG80.24316109422492402No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCCCCACGTTGC70.2127659574468085No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG70.2127659574468085No Hit
GACATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC60.182370820668693No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG60.182370820668693No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG60.182370820668693No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCTACGTTGC50.1519756838905775No Hit
GTCATGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG50.1519756838905775No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT50.1519756838905775No Hit
ATCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC50.1519756838905775No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT50.1519756838905775No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT50.1519756838905775No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG40.12158054711246201No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC40.12158054711246201No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG40.12158054711246201No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT40.12158054711246201No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTATCCACGTTGC40.12158054711246201No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTA40.12158054711246201No Hit
GCACAGGTCGTATACGCATCTCCGAGCCCACGAGACGGATAACAATCTCG40.12158054711246201No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA40.12158054711246201No Hit
GTCATTGTCACTGTTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC40.12158054711246201No Hit
TCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG40.12158054711246201No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC40.12158054711246201No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT40.12158054711246201No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCATGTTGC40.12158054711246201No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG40.12158054711246201No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGGA40.12158054711246201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)