FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741
Sequences flagged as poor quality0
Sequence length150
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG59334.0608845491097No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18110.396323951751867No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG1408.0413555427915No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT1227.00746697300402No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC553.159103963239517No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG442.527283170591614No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT181.0338885697874785No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC181.0338885697874785No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTA181.0338885697874785No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT130.7466973004020678No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC120.6892590465249857No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.6318207926479035No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT110.6318207926479035No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC110.6318207926479035No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.6318207926479035No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG80.4595060310166571No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA70.402067777139575No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGT70.402067777139575No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.34462952326249285No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.34462952326249285No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC50.2871912693854107No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG50.2871912693854107No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG50.2871912693854107No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG40.22975301550832855No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT40.22975301550832855No Hit
GCACAGGTCGTATACGCATTGGCGGCAGTACTCAGAGGGACTCAGCACCG40.22975301550832855No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT40.22975301550832855No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT40.22975301550832855No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT40.22975301550832855No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG40.22975301550832855No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC30.17231476163124643No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT30.17231476163124643No Hit
CCTTTAACAAGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.17231476163124643No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG30.17231476163124643No Hit
TCTCGTGCTCGCAGATGCCGCCCAGTGGCTCCGGGGCGGCAGGTGGGGCG30.17231476163124643No Hit
CAAGCAATCAAGGCCAGTCTACACATCTCCGAGCCCACGAGACGTGCATT30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGGAGAC30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGAC30.17231476163124643No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACG30.17231476163124643No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG30.17231476163124643No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGGGACGT30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGAC30.17231476163124643No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACG20.11487650775416428No Hit
TCCCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG20.11487650775416428No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAACCCACGAGAC20.11487650775416428No Hit
CACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGC20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG20.11487650775416428No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACATCATCTCATCTCGT20.11487650775416428No Hit
ACACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG20.11487650775416428No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACAAGAC20.11487650775416428No Hit
GCACAGATCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGTGGAAGTACTCAGAGGGACTCAGCACCG20.11487650775416428No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAAGAC20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGTACCG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACCCAGCACCG20.11487650775416428No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGCGACGT20.11487650775416428No Hit
GCACAGGTCGTATACACATTGGCGGAAGTACTCAGAGGGACTCAGCACCG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAAACATCTCCGAGCCCACGAGAC20.11487650775416428No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATTCCGAGCCCACGAGACGTG20.11487650775416428No Hit
AGAAAAAAAGTTTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTA20.11487650775416428No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCT20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCAAGCCCACGAGAC20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCA20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGAC20.11487650775416428No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCTGAGCCCACGAGAC20.11487650775416428No Hit
GCACAGGTCGTATACGCATCTCCGAGCCCACGAGACGTGCATTCATCTCG20.11487650775416428No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAATCTCCGAGCCCACGAGACGTG20.11487650775416428No Hit
GCACAGGCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGC20.11487650775416428No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTATTCAGAGGGACTCAGCACCG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACG20.11487650775416428No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGTCCACGAGACGT20.11487650775416428No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG20.11487650775416428No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACG20.11487650775416428No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAAGAC20.11487650775416428No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC20.11487650775416428No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT20.11487650775416428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)