FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8789
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC345239.27636818750711No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG107012.174308795084766No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG7568.601661167368302No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC5916.7243144840141085No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG4965.643417908749574No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT3383.845716236204346No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG2612.969621117305723No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG2032.3097053134600065No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA1511.7180566617362614No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC1361.5473887814313347No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG510.5802707930367506No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT370.42098077141881896No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGC210.23893503242689726No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCCCCACGTTGC190.21617931505290705No Hit
GTCATTTTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC180.20480145636591193No Hit
GTCATTGTCACTGGTCAGCCCCAGCACCTTCTGCTGCGTCTCCACGTTGC170.19342359767891684No Hit
ATCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC140.15929002161793152No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT120.13653430424394128No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA110.1251564455569462No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC100.11377858686995107No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCCGCGTCTCCACGTTGC90.10240072818295597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)