FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149704
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG61624.116122481697216RNA PCR Primer, Index 46 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC32422.1656067974135627No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC28281.88906108053225No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC25201.683321754929728No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGC23271.5544006840164593No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG23231.55172874472292RNA PCR Primer, Index 46 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG23231.55172874472292RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20321.3573451611179395RNA PCR Primer, Index 46 (96% over 28bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT13830.923823010741196No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13500.9017795115694971No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12750.8516806498156362No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA12730.8503446801688667No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA10850.7247635333725219No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT10820.7227595789023673No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG10640.7107358520814407RNA PCR Primer, Index 46 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA10170.6793405653823545No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT9850.6579650510340405No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT9510.6352535670389569No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8790.5871586597552504No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA8440.563779190936782No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGC7930.5297119649441565No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG7850.524368086357078TruSeq Adapter, Index 4 (96% over 27bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC7150.4776091487201411No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG6980.46625340672259924No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA6790.4535616950782878No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA6200.4141505904985839No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG6100.40747074226473573No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT5900.3941110457970394No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG5810.3880991823865762No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG5610.37473948591887996No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG5240.35002404745364185No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG5120.3420082295730241No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC4620.3086089884037834No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA4480.29925720087639607No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT4400.29391332228931755No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC4190.2798856409982365No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG4030.2691978838240795No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA3900.26051408112007696No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3700.24715438465238068No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA3620.2418105060653022No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA3510.23446267300806928No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3240.21642708277667932No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG3220.21509111312990967No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT3070.2050713407791375No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA2980.1990594773686742No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC2950.1970555228985197No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG2890.19304761395821088RNA PCR Primer, Index 46 (96% over 28bp)
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT2790.18636776572436273RNA PCR Primer, Index 46 (95% over 21bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG2760.18436381125420828No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT2740.18302784160743868RNA PCR Primer, Index 46 (95% over 21bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2640.17634799337359056RNA PCR Primer, Index 46 (96% over 28bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2640.17634799337359056No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT2620.17501202372682093No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2540.16966814513974243No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2530.16900016031635762No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG2430.1623203120825095No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA2390.15964837278897023No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG2380.15898038796558545No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG2360.1576444183188158No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC2250.15029658526158285No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG2230.14896061561481322RNA PCR Primer, Index 46 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG2220.14829263079142843No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2210.14762464596804362No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG2200.14695666114465877No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2190.14628867632127399No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG2170.14495270667450436No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT2100.14027681291081065No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2060.13760487361727142No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG2030.13560091914711697RNA PCR Primer, Index 46 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG1950.13025704056003848No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG1880.12558114679634477No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC1880.12558114679634477RNA PCR Primer, Index 46 (95% over 22bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1830.12224122267942072RNA PCR Primer, Index 46 (96% over 28bp)
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG1810.1209052530326511No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT1770.11823331373911185No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1760.11756532891572705No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC1740.1162293592689574No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1740.1162293592689574No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1720.1148933896221878No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1690.11288943515203335No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCC1690.11288943515203335No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG1640.10954951103510928No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT1640.10954951103510928No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT1590.10620958691818522No Hit
GCGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGA1590.10620958691818522No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG1580.1055416020948004RNA PCR Primer, Index 46 (96% over 28bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA1560.10420563244803079No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA1560.10420563244803079No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1560.10420563244803079No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG1540.10286966280126114RNA PCR Primer, Index 46 (96% over 28bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC1540.10286966280126114No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1540.10286966280126114No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT1530.10220167797787634No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA1530.10220167797787634No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1530.10220167797787634No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCAC1520.10153369315449154No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1510.10086570833110671RNA PCR Primer, Index 46 (96% over 28bp)
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1500.1001977235077219RNA PCR Primer, Index 46 (96% over 28bp)
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1500.1001977235077219RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)