FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403070
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG267391.1127016691149239RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT139100.5788428967945171No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC90320.37585255527304656No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATG84720.3525490310311393No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG82370.3427698735367675RNA PCR Primer, Index 17 (96% over 28bp)
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAAT81920.34089726891018574No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG80770.3361117237533655RNA PCR Primer, Index 17 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC76480.31825955964661873No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGC65790.2737747964062637No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCAAGCCCCACCAA58230.2423150386796889No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC57730.24023436687237576No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT57080.23752949352286867No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTT56560.23536559484326297No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG54530.22691806730557162RNA PCR Primer, Index 17 (96% over 28bp)
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA52550.21867860694861158No Hit
GTTGTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACGG49910.20769265980599816No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC47290.19678993953567728No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC46970.19545830957899685No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG44510.1852214042870162No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA43470.18089360692780485No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTA41120.17111444943343307No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC40450.16832634921163347No Hit
AAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT37820.1573820155051663No Hit
CTTGTGGACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGTTGTAGACGGCCTTGGTAGG35850.14918416858435252No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG34040.14165213664187892No Hit
GTCTACAACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCAAGCCCCACCAAGCAGTCCACAAGCACGCCCGCTGC32460.1350772137307694No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG32430.1349523734223306No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAG31220.12991714764863277No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA31090.12937617297873139No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGC30550.12712904742683317No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAA29110.12113671262177132No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC27660.1151027643805632No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT27540.11460340314680806No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGT26830.11164884918042338No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCG26510.11031721922374296No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATC25840.10752911900194335No Hit
GCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACGGACAAGGGGAA25260.1051155397054601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)