FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850165
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC33300.17998394737766632No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC26740.14452765023660052No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG26100.14106849929600873TruSeq Adapter, Index 3 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC21580.11663824577807926No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC18960.10247734661503163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)