FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455534
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA338300.7592804813070667No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT177860.39918896365733036No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA151990.3411263386162018No Hit
GTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCA146110.32792926728872457No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG145340.326201079376793No Hit
GCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAG129580.29082933717933696No Hit
TTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAG126910.2848367894847172No Hit
TCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGG123380.27691405788845963No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA112560.25262965112599295No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTC108640.2438316035743415No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA106250.2384674878476968No Hit
GGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCT104940.23552732399752754No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCT104540.2346295640432774No Hit
TACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAAT104500.23453978804785242No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG90880.20397106160563472No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGC89480.20082890176575918No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC88660.19898849385954634No Hit
GGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATA88540.1987191658732713No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC86740.1946792460791456No Hit
GACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG86360.19382637412260798No Hit
CCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGC85840.19265928618208278No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC84070.18868669838452587No Hit
AAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC83420.18722783845886937No Hit
CCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGG82520.1852078785618065No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATGCTCTTCCAGC79770.1790357788763367No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC78390.17593850703417369No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT73680.16536738357287814No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC73060.1639758556437904No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAA72920.16366163965980285No Hit
GGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACA69300.15553691207383896No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC69030.15493092410472012No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGC68020.15266408022023847No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCT65200.14633487254277489No Hit
GTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATG65120.14615532055192487No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACAT64960.14579621657022482No Hit
ACCGAGTACCGTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA64690.14519022860110595No Hit
GCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT64470.14469646062626837No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC63250.1419582927658054No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCC62540.14036476884701138No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACG62250.13971389288018002No Hit
GGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATG60510.13580863707919186No Hit
TCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT59130.1327113652370288No Hit
GAGTACGACCGGGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA58530.1313647253056536No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGG58310.13087095733081602No Hit
GCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCG57400.12882855343489694No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC57180.12833478546005933No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGC56680.12721258551724665No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATG56130.12597816558015268No Hit
GTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAATCTGGAGGACCGAGTACCGTCCATGCCCGGTCGTA55570.12472130164420246No Hit
ATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG55390.1243173096647899No Hit
GGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGA55100.12366643369795854No Hit
ACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGA55070.12359910170138977No Hit
GTATCAAGGGTGCACTATGCACGAACATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGA54590.12252178975628959No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT54460.1222300177711583No Hit
TCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAGCCCACGG54340.12196068978488324No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATGTGATTT53860.12088337783978306No Hit
GGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTG53450.11996317388667667No Hit
GAGTTATCTTTTCTTTTTAACAGCTTGCCCACCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAG53120.11922252192442027No Hit
GAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAAT52880.1186838659518702No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC52400.11760655400677No Hit
GTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACT52330.11744944601477622No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC52140.1170230100365074No Hit
GTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGTACTCG51640.11590081009369471No Hit
CATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC51610.11583347809712595No Hit
GGTTAATATTCCCGAACCGGGACATGGCGGCGGACGGCAACGTTAGGAAATCCGGAGACGTCGTCGGGGGCCCCG51470.1155192621131384No Hit
AGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCT51050.11457661416117573No Hit
GGTCAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGG50640.11365641020806934No Hit
GCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCG47890.10748431052259952No Hit
AGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGGGTCT47810.10730475853174952No Hit
ATTTCAGGGTGGGCAAGCTGTTAAAAAGAAAAGATAACTCTTCCCGGGGCCCCCGACGACGTCTCCGGATTTCCT47680.1070129865466182No Hit
ACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGT47590.10681099055691193No Hit
GGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGG47000.10548679462439295No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC46820.10508280264498036No Hit
GGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGA45730.10263640676964872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)