FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269845
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC12700.47064055291000395No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC11160.41357075358075934No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC11130.41245900424317666No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC8300.30758398339787657No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG8220.30461931849765606TruSeq Adapter, Index 11 (96% over 28bp)
GAATAGGACGAGGACGATGGGCGTGTGCTGGGCAGGGAATATTACAATGAGGGGGTGCAGGGTAGCCCGGCTTGT7740.2868313290963331No Hit
GTCTAACGAAGAGAGCTTAAATGAAAGTGTGAATGGAGGATTGCCAACAGGGTTGACAAAGGGTGTATCGTGCTG7330.27163742148270303No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC7070.26200226055698644No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC6650.24643776983082882No Hit
GGATAAGAGGGCGATGATTTCAGTTTAAACATGCAGAATAGGACGAGGACGATGGGCGTGTGCTGGGCAGGGAAT5580.20678537679037967No Hit
TAGCAATACTGTTGTTAAGTTCCACGATTCTGAAAACAGACGCTGAAGTTGCTGCCGCAGAGTCTAACGAAGAGA4730.17528581222553688No Hit
GTGCAGGGTAGCCCGGCTTGTGGCAGCACGATACACCCTTTGTCAACCCTGTTGGCAATCCTCCATTCACACTTT4500.16676240063740294No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG4440.16453890196223758RNA PCR Primer, Index 11 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4370.16194482017454465No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAG4280.1586095721617966No Hit
GGGTAGCCCGGCTTGTGGCAGCACGATACACCCTTTGTCAACCCTGTTGGCAATCCTCCATTCACACTTTCATTT4230.15675665659915877No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4150.15379199169893829No Hit
TCGTTAGACTCTGCGGCAGCAACTTCAGCGTCTGTTTTCAGAATCGTGGAACTTAACAACAGTATTGCTATTGCT4020.14897441123608No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3910.1448979969982768No Hit
GAATATTACAATGAGGGGGTGCAGGGTAGCCCGGCTTGTGGCAGCACGATACACCCTTTGTCAACCCTGTTGGCA3790.14045099964794602No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG3660.13563341918508773No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAG3450.12785117382200895No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT3430.1271100075969538No Hit
GTAATATTCCCTGCCCAGCACACGCCCATCGTCCTCGTCCTATTCTGCATGTTTAAACTGAAATCATCGCCCTCT3340.12377475958420575No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT3270.1211806777965128No Hit
GAGGATAAGAGGGCGATGATTTCAGTTTAAACATGCAGAATAGGACGAGGACGATGGGCGTGTGCTGGGCAGGGA3210.11895717912134744No Hit
CTACATAGAGTATCAAAGGAAATGAGGATAAGAGGGCGATGATTTCAGTTTAAACATGCAGAATAGGACGAGGAC3160.11710426355870963No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG3150.11673368044618207RNA PCR Primer, Index 11 (96% over 28bp)
AACGAAGAGAGCTTAAATGAAAGTGTGAATGGAGGATTGCCAACAGGGTTGACAAAGGGTGTATCGTGCTGCCAC2990.11080435064574107No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG2990.11080435064574107No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA2980.11043376753321352No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG2960.10969260130815837No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGA2900.10746910263299302No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG2870.10635735329541034No Hit
GACAAAGGGTGTATCGTGCTGCCACAAGCCGGGCTACCCTGCACCCCCTCATTGTAATATTCCCTGCCCAGCACA2830.10487502084530007No Hit
TCTGAAAACAGACGCTGAAGTTGCTGCCGCAGAGTCTAACGAAGAGAGCTTAAATGAAAGTGTGAATGGAGGATT2780.10302210528266226No Hit
CCCTTTGTCAACCCTGTTGGCAATCCTCCATTCACACTTTCATTTAAGCTCTCTTCGTTAGACTCTGCGGCAGCA2750.10191035594507958No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAG2720.1007986066074969No Hit
ATTCTGAAAACAGACGCTGAAGTTGCTGCCGCAGAGTCTAACGAAGAGAGCTTAAATGAAAGTGTGAATGGAGGA2710.10042802349496933No Hit
GTTGTTAAGTTCCACGATTCTGAAAACAGACGCTGAAGTTGCTGCCGCAGAGTCTAACGAAGAGAGCTTAAATGA2710.10042802349496933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)