FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085120
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA338590.8288373413755287No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT169460.41482257559141467No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG147520.3611154629484568No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA143200.3505404981983393No Hit
GTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCA136480.33409055303148993No Hit
GCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAG124190.3040057574808084No Hit
TCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGG116970.2863318580604732No Hit
TTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAG115330.28231728810903967No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA107120.2622199592668024No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA102660.2513022873257089No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCT102040.24978458405138648No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTC99650.24393408271972425No Hit
GGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCT99560.24371377095409683No Hit
TACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAAT94550.23144974933416887No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC85020.20812118126272913No Hit
GACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG82620.2022462008459972No Hit
GGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATA80790.19776652827823907No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGC80490.19703215572614757No Hit
AAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC80480.19700767664107785No Hit
CCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGG76120.1863347955506815No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG75560.18496396678677737No Hit
CCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGC75400.18457230142566192No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT72830.1782811765627448No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATGCTCTTCCAGC69220.16944422685257715No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC68650.16804891900360333No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAA68220.16699631834560552No Hit
GGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACA65330.15992186276045744No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGC62090.15199063919786934No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACAT61540.15064428951903494No Hit
GCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT60820.14888179539401536No Hit
GTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATG60550.148220860097133No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC59630.1459687842707191No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACG59480.14560159799467334No Hit
ACCGAGTACCGTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA57040.13962870123766255No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCT56620.13860057966473444No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCC56270.13774381168729438No Hit
GAGTACGACCGGGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA54380.13311726460911796No Hit
TCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT53930.13201570578098074No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATG53160.13013081623061257No Hit
GGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATG52710.12902925740247534No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGC52700.12900477831740562No Hit
ATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG52270.1279521776594078No Hit
GCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCG52060.12743811687294376No Hit
GTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAATCTGGAGGACCGAGTACCGTCCATGCCCGGTCGTA51090.12506364562118125No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATGTGATTT51060.1249902083659721No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGG50440.1234725050916497No Hit
TCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAGCCCACGG49920.12219959266802445No Hit
CATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC49410.12095115932946891No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC49220.12048605671314427No Hit
GTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACT48680.1191641861193796No Hit
GGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGA48430.1185522089926367No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC48230.11806262729124237No Hit
AGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCT47910.11727929656901144No Hit
ACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGA47250.11566367695441016No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT47230.11561471878427072No Hit
GAGTTATCTTTTCTTTTTAACAGCTTGCCCACCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAG47080.11524753250822498No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC46650.11419493185022717No Hit
GGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTG46480.11377878740404199No Hit
GTATCAAGGGTGCACTATGCACGAACATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGA46190.11306889393702022No Hit
GTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGTACTCG46020.11265274949083504No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC45560.11152671157762808No Hit
GAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAAT45550.11150223249255836No Hit
GCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCG44960.11005796647344508No Hit
GGTTAATATTCCCGAACCGGGACATGGCGGCGGACGGCAACGTTAGGAAATCCGGAGACGTCGTCGGGGGCCCCG44330.10851578411405297No Hit
GGTCAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGG43830.10729182986056714No Hit
GGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGA42650.1044032978223406No Hit
GGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGG42290.10352205075983081No Hit
ACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGT41400.10134341218862603No Hit
AGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGGGTCT41300.10109862133792888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)