FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002296824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4927139
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA407110.8262604322711414No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC218610.4436854734562999No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT202410.4108063523273851No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG179610.3645320337015051No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA166050.3370109915713764No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC151250.30697327597212093No Hit
GTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCA150300.3050451793627093No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC146960.2982663975990935No Hit
GCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAG143990.2922385587254591No Hit
TCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGG132460.26883755461333647No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC127680.25913618430492824No Hit
TTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAG126960.2576748900325321No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA124970.2536360350296592No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA122420.24846061781492262No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCT112530.228388117323258No Hit
GGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCT111200.22568878207008164No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTC110510.22428837505903526No Hit
TACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAAT104730.21255742937229902No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC104160.21140057140665203No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC98980.20088737094691259No Hit
GACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG98680.20027849833341416No Hit
AAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC96760.19638171360702428No Hit
GGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATA94900.19260670340333408No Hit
CCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGG88170.17894766110718613No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGC87080.17673542394480854No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT85230.1729807094949016No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC85040.17259509017301927No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC83780.1700378251963259No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG83480.16942895258282747No Hit
GGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACA81880.16618163197750258No Hit
CCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGC80110.16258928355786187No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATGCTCTTCCAGC77380.15704854277502625No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAA73880.149945028950878No Hit
GCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT73510.1491940860608966No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGC72300.14673829985311962No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACG71120.1443434009066925No Hit
GTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATG70230.1425370788199805No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACAT67890.13778787243469282No Hit
TCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT64670.13125263971647644No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCC63650.1291824728305818No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATG63590.1290606983078821No Hit
ATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG63160.12818798089520106No Hit
ACCGAGTACCGTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA62720.1272949677287367No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCT61770.125366871119325No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATGTGATTT60940.12368232355531274No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGC60310.12240369106696605No Hit
GGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATG60300.12238339531318278No Hit
GTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACT58910.11956228553730675No Hit
GCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCG58760.11925784923055753No Hit
GAGTACGACCGGGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA58110.1179386252346443No Hit
CATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC58030.11777625920437802No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC55950.11355474241745565No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGG55790.11323001035692315No Hit
GTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAATCTGGAGGACCGAGTACCGTCCATGCCCGGTCGTA55180.11199196937614303No Hit
GGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGA55010.11164694156182725No Hit
TCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAGCCCACGG54660.11093659017941243No Hit
AGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCT54190.10998268975159825No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT53270.10811548040353641No Hit
ACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGA52940.10744572052868814No Hit
GGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGA52060.10565969419575945No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC51510.104543427737679No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC50700.10289947168123327No Hit
GAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAAT50420.1023311905753014No Hit
GCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCG50120.10172231796180299No Hit
GAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGT50040.10155995193153675No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA50000.10147876891640362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)