FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002296829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973625
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC443781.4923872377989826No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA276860.9310521669679264No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC243060.8173861868931018RNA PCR Primer, Index 45 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC214800.7223506662743285RNA PCR Primer, Index 45 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC209990.706175122955988No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC186980.6287948211358191RNA PCR Primer, Index 45 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC166870.5611669258901173RNA PCR Primer, Index 45 (95% over 21bp)
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG105660.3553238891924839No Hit
GTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCA103430.34782462482660054No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA100460.33783681533481863No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT99270.3338349657404683No Hit
GCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAG92810.3121106393711379No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA89460.3008449283282189No Hit
TTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAG81510.2741098827189037No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCA80570.2709487578292488No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC77420.26035562655008615RNA PCR Primer, Index 45 (95% over 21bp)
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCT76780.25820337130606585No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTC75960.25544579427466474No Hit
TCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGG70650.23758880154693346No Hit
GGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATA67600.227331960149649No Hit
GACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG66900.22497793097650173No Hit
TACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAAT66240.22275841775610578No Hit
GGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCT65940.22174954811047123No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG65490.2202362436420194RNA PCR Primer, Index 45 (96% over 30bp)
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC64190.2158644751776031No Hit
AAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC63200.21253520534700912No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT57280.19262684433982094No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATGCTCTTCCAGC54660.18381604943461263No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG54390.18290806675354154No Hit
CCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGG54170.18216822901340957No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA53780.18085669847408464No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGC52020.17493799655302872No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC51720.17392912690739418No Hit
GGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACA51120.1719113876161251No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGC50650.17033082517129766No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAA50010.1681785699272773No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATT47390.15936777502206903No Hit
CCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGC46800.15738366471898776No Hit
GCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT46770.1572827777544243No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACG46510.15640842406154104No Hit
ACCGAGTACCGTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA44520.1497162554121653No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACAT42960.14447013325486568No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC42590.14322586069191642No Hit
GTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATG42570.1431586027155408No Hit
GGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATG41240.13868594728656103No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGG39980.13444869477489596No Hit
GTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACT39650.13333893816469797No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC39310.13219555256631216No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATGTGATTT38960.13101853797973853No Hit
GGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGA38780.13041321619235782No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCC38610.1298415233931649No Hit
ATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG38560.12967337845222582No Hit
GCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCG38310.12883265374753036No Hit
GAGTACGACCGGGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA37870.12735297826726638No Hit
GTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAATCTGGAGGACCGAGTACCGTCCATGCCCGGTCGTA37280.12536886796418512No Hit
GTATCAAGGGTGCACTATGCACGAACATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGA36850.12392282147210897No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATG36480.12267854890915969No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGC36390.12237588801546934No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCT36240.12187145319265207No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG36050.12123250241708353No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT35920.1207953255706419No Hit
GAGTTATCTTTTCTTTTTAACAGCTTGCCCACCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAG34980.11763420068098701No Hit
TCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT34730.11679347597629156No Hit
ACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGA34230.11511202656690066No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT33950.11417041489764176No Hit
CATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC33610.11302702929925595No Hit
GCCCACGGGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTT33470.11255622346462651No Hit
TCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAGCCCACGG33320.11205178864180924No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC33180.11158098280717979No Hit
GGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGA33060.11117743494892598No Hit
GAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAAT33000.11097566101979905No Hit
GTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGTACTCG32700.10996679137416451No Hit
GAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGT32440.10909243768128125No Hit
GGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGG31910.10731010130732692No Hit
GAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACATTGTTCTATTGACCAGAGG31810.10697381142544873No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC31630.106368489638068No Hit
CCGCAAGACGTGCGATGCTCTTCCAGCTGCTGGACCCTATCTCCGGTTGAGCCGATTTCAGGGTGGGCAAGCTGT31210.10495607213417965No Hit
GGTTAATATTCCCGAACCGGGACATGGCGGCGGACGGCAACGTTAGGAAATCCGGAGACGTCGTCGGGGGCCCCG30960.10411534742948421No Hit
AGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCT30840.1037117995712304No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC30540.10270292992559586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)