FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4555541
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC863451.8953841047638469No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC530781.1651305520024955No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC481761.0575253301419085No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC413850.9084541221339025No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA409030.8978736005229675No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC354980.7792268799688116No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC295330.6482874372110798No Hit
GCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAG162050.3557206487659753No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA161920.35543528200053515No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG158110.3470718406441738No Hit
GTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCA152600.33497668004744113No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC146440.3214546856235077No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT141720.31109367690906525No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA127140.27908869660046964No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATA117110.25707155308227936No Hit
TTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAG116520.25577642699297404No Hit
GGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCT112390.2467105443678369No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTC111310.24433980508571868No Hit
TCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGG111060.24379102284448764No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCT110010.24148613743131714No Hit
GGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATA101990.22388120313262463No Hit
GACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG98630.21650556981047914No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG96740.2123567760667723No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG95670.21000798807430335TruSeq Adapter, Index 6 (96% over 28bp)
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC94770.20803237200587152No Hit
AAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC94060.2064738304407753No Hit
TACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAAT91770.20144698511109876No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATGCTCTTCCAGC84980.18654205943926308No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT82070.18015423415133353No Hit
CCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGG80540.17679568683499938No Hit
ACCGAGTACCGTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA79980.1755664146146418No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGC79050.17352494467726226TruSeq Adapter, Index 6 (96% over 25bp)
GAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC77450.17001273833338346No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGC74930.16448101334177434No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGC74410.1633395462800137No Hit
GGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACA74120.1627029588801857No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA73870.16215417663895462No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGA72350.15881758061226975No Hit
CCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGC71680.1573468442057705No Hit
GCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT70900.1556346436131296No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAA69120.15172731405556442No Hit
GTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATG67430.14801754610484244No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACG66210.14533948876763483No Hit
GGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGATG64350.14125654889287573No Hit
CAATAGAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCT64310.14116874373427876No Hit
GTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAATCTGGAGGACCGAGTACCGTCCATGCCCGGTCGTA64250.14103703599638331No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC62180.13649311903899009No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC61360.13469311328775221No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGCGAGAGGACAT61360.13469311328775221No Hit
GTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACT60970.13383701299143175No Hit
GGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGA60360.13249798432282794No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGG60190.13212481239879084No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT60160.13205895852984312No Hit
GAGTACGACCGGGCATGGACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA60130.13199310466089537No Hit
GGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCGGCGAGGG57370.12593454871770443No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC54880.12046867759504304No Hit
GCCTAGGGAAAAGCCCCCGCGAGAGGACATTCTACGTCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCG54800.12029306727784912No Hit
GTATCAAGGGTGCACTATGCACGAACATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGA54650.11996379793311046No Hit
ATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG54590.11983209019521503No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCC53860.11822964605082031No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATGTGATTT53550.11754915607169378No Hit
GCCCACGGGTTGAGGACGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTT53460.1173515944648506No Hit
ACCGGAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGA53430.11728574059590288No Hit
GAGTTATCTTTTCTTTTTAACAGCTTGCCCACCCTGAAATCGGCTCAACCGGAGATAGGGTCCAGCAGCTGGAAG52580.11541988097571727No Hit
GAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACATTGTTCTATTGACCAGAGG51900.11392719327956878No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGCCGCCTAGGGAAAAGCCCCCGC51710.11351011877623317No Hit
GAGATAGGGTCCAGCAGCTGGAAGAGCATCGCACGTCTTGCGGTGTCCGGTGCGCCCTCGGCGGCCCTTGAAAAT51460.11296133653500211No Hit
GCTCTGAGGGCTGGGCCTAGGGGTCTGCGTCCTCAACCCGTGGGCTGTCGGCAGTCTGCCCGAGCTGCTATCGCG50060.10988815598410814No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTGACGCAATG49440.1085271760258551No Hit
CCGCAAGACGTGCGATGCTCTTCCAGCTGCTGGACCCTATCTCCGGTTGAGCCGATTTCAGGGTGGGCAAGCTGT48790.10710034219865434No Hit
TCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGATAGCAGCTCGGGCAGACTGCCGACAGCCCACGG47960.10527838515776722No Hit
CATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC47910.10516862870952101No Hit
GGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGA47900.10514667741987176No Hit
GTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGGACGGTACTCG47580.10444423615109599No Hit
TCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT47290.10380764875126797No Hit
GAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGT46430.10191983784143309No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT46070.10112959141406036TruSeq Adapter, Index 6 (95% over 21bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG45680.10027349111773991No Hit
GGTTAATATTCCCGAACCGGGACATGGCGGCGGACGGCAACGTTAGGAAATCCGGAGACGTCGTCGGGGGCCCCG45660.10022958853844142No Hit
AGCTGCTATCGCGGCGAGGGCGGGCAGTCGCGTGCCGATTGGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCT45560.10001007564194901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)