FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375041
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC550624.004389687289325No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC225001.6363148444300935No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC148971.0833858772211156No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC148851.082513175970753No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC144351.049786879082151No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90940.6613620975665453TruSeq Adapter, Index 12 (96% over 29bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA88020.6401263671410525No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC75560.5495108873117238No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGT71780.5220207979252983No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG34000.24726535426943635RNA PCR Primer, Index 12 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG31680.23039313009575713RNA PCR Primer, Index 12 (96% over 29bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC31020.2255932732187622No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGT29560.21497540800601583No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG28290.2057393197730104No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG25190.18319453747197356No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGT24370.17723107892782833No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG23260.16915859236197323TruSeq Adapter, Index 12 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG21760.15824982673243926No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT19310.14043217620420045TruSeq Adapter, Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG18230.13257786495093601TruSeq Adapter, Index 12 (96% over 29bp)
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC17030.12385085244730884No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG15230.11076033369186811No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAAT14690.10683317806523587No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA14410.10479687514772287No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGC14350.1043605245225415No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGT14290.10392417389736015No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14240.1035605483763757No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG14060.10225149650083161TruSeq Adapter, Index 12 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG14030.10203332118824095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)