FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174585
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC79894.575994501245812No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC60383.4584872698112665No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTA47582.7253200446773778No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATA35862.0540138041641605No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT35322.0230833118538247No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG33851.9388836383423547Illumina PCR Primer Index 6 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC32901.8844688833519487No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC32171.8426554400435318No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC29301.6782656012830428No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT21541.2337829710456225No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT18811.0774121488100352No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT16450.9422344416759744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG13460.7709711601798551Illumina PCR Primer Index 6 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13060.748059684394421No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG11870.6798980439327548No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC11530.660423289515136No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT10730.6146003379442678No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG10110.5790875504768451No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10100.5785147635822092No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC9720.556748861586047No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATC8800.5040524672795487No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT8220.4708308273906693No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7850.4496377122891428No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA7750.44390984334278427No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT7580.43417246613397487No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG7460.42729902339834464No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT7360.4215711544519861No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT6850.3923590228255578No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA6720.3849127931952917No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT6510.3728842684079388No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG6100.34940000572786895Illumina PCR Primer Index 6 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC5860.33565312025660854No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT5490.31446000515508205No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC5330.30529541484090844No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA5250.30071311968382164No Hit
ATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG5100.29212131626428384Illumina PCR Primer Index 6 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC4900.28066557837156686No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT4890.280092791476931No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA4850.27780164389838763No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT4670.26749147979494226No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG4620.2646275453217631No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT4520.2588996763754045No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG4380.2508806598505026Illumina PCR Primer Index 6 (96% over 27bp)
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT4330.24801672537732333No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG4270.24458000400950827No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTG4270.24458000400950827No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT3790.21708623306698743No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCT3720.21307672480453646No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCC3520.20162098691181946No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG3480.19932983933327605No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA3420.19589311796546094No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT3260.18672852765128733No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA3250.18615574075665148No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCC3240.18558295386201562No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC3180.18214623249420053No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC3070.17584557665320616No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA3040.17412721596929862No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGA2960.1695449208122118Illumina PCR Primer Index 6 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT2940.16839934702294013No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG2930.16782656012830427No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCT2820.16152590428730992No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2770.15866196981413067No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCC2720.15579803534095138No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT2720.15579803534095138No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCT2690.15407967465704384No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCG2660.1523613139731363No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT2640.1512157401838646No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2600.1489245926053212No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG2590.14835180571068535No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT2490.14262393676432683No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2470.14147836297505514No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT2460.1409055760804193No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG2450.14033278918578343No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATG2420.13861442850187589No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA2360.13517770713406077No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG2340.13403213334478906Illumina PCR Primer Index 6 (96% over 27bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATA2260.12944983818770225No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2210.126585903714523No Hit
ACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG2180.12486754303061545Illumina PCR Primer Index 6 (96% over 27bp)
GACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG2170.1242947561359796No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGA2160.12372196924134375Illumina PCR Primer Index 6 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA2140.12257639545207206No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT2070.1185668871896211Illumina PCR Primer Index 6 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC2060.11799410029498525No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2020.11570295271644185No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA2000.11455737892717013No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCG2000.11455737892717013No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTA1970.1128390182432626No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCA1960.11226623134862675No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTC1940.11112065755935505Illumina PCR Primer Index 6 (95% over 21bp)
GTGTATAAGAGACAGCTGAGGACGCTGCGGATGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCAC1910.10940229687544749No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG1900.10882950998081165No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC1890.10825672308617579No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG1880.10768393619153993No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCT1870.10711114929690409Illumina PCR Primer Index 6 (96% over 25bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA1860.10653836240226823No Hit
ATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCT1860.10653836240226823No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC1860.10653836240226823No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC1830.10482000171836067No Hit
AGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT1820.10424721482372483No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCAC1800.10310164103445314No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG1800.10310164103445314No Hit
GCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG1790.10252885413981729Illumina PCR Primer Index 6 (95% over 23bp)
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTAT1760.10081049345590973No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1760.10081049345590973No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGCCGTC1760.10081049345590973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)