FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197084
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC89130.7445592790480868No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG53870.45001019143184606RNA PCR Primer, Index 32 (96% over 33bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC30730.2567071316632751RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC28900.24141998389419625RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC21950.18336223690233935RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC21540.17993724751145282No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG18920.1580507299404219RNA PCR Primer, Index 32 (96% over 33bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG16270.13591360338957No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA14820.12380083603155667No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATT12850.10734417968997999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)