FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAGTTCAAGACCAGCCTGGCTAATGTCTGTCTCTTATACACATCTGACGCTGCC13.125No Hit
GCTGTAGGGTGGCTCAGTGGAATCCATTTTGTTAACACCGACAATTAGTTGTTTCACACCCAGTGTGTAAGCCTG13.125No Hit
CTCCTATACAGCTAGGCCCCAGGGTTGGGAGAGTTCTTTCCCTGCTCATGGCTGAATTTCCCGAGAGCCAGGCTG13.125No Hit
CAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAT13.125Clontech Universal Primer Mix Short (95% over 22bp)
GCCTTGGGCTTTTGGATCGTCGCCGACTCCTAGACTTAGAATGTGACCGCCGTCTCGATCTGCTTCTTGACCGCC13.125No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGA13.125No Hit
GGTATACACGAAGATTAAATTTTTCATAGTCATCAAGAAATGTATAGTAGAAAACTATTACTAACACGAAGATAA13.125No Hit
GTGTTATAGCACCCTACTTCCTGCAGCAACGGGACACCCTGCGGCGCCATGTGCAGAAACAGGAGGCCGAGAACC13.125No Hit
GCTTGGCCCAGTGCCGCCACCACTGGGGAGTGGGAGGCTCAGGCAGACCTAGGGCCCTGGGACCGTGGCCCTTGG13.125No Hit
GTCCAGAGGTTCCATAGCTTTGTTGCCCATATGCCTGGGTGGTCTGTGCATATCCTTGAGTGGGCTGGGCGGTGT13.125No Hit
GAGCGAAAAAGTGAATATTACATACAGCTACTGATCAAATCCATCCAAACGACTCTGATATGCTACACTGACTCT13.125No Hit
GGGCTGCTCAAGCTGGCGTCGCCCGAGCTGGAGCGCCTGATAATCCAGTCCAGCAACGGGCACATCCTGTCTCTT13.125No Hit
AGGTTCTGATAGCCCTATACCCCAAACACCCATTTAAGATGCGTTGAAACCATTAAAATTATACACAAATGCCGA13.125No Hit
ACCATACATGGTCTTCTGACTCAAGTTGGAGAAGAGCATTCAAGTGTGGTGTAGATTACATCTGTCTCTTATACA13.125No Hit
ACATGCTGAAGCAAAGTGCTAACATGCATTGGTTCAAGGGATGCAAAGACACCCTTAAGGATGGCAATGCCAGTG13.125No Hit
GTATCAACGCAGAGTACATGGGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAAAAATGAATGG13.125No Hit
GCAAGAGACAGACCCGCGGGACCGCCGAACTGCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCGA13.125No Hit
CCCCAGCAACCAGAGCGAAGGCATGAATAGAACAGAAATCTACTGATACACACCCGCACATATACTCTGCGTTGA13.125No Hit
CTCCTGTGTGCGTCCGGTGGTGTTCGATAAGTGCTGAGCTGTGACTAAAGGCCTTTCCGCATTCCTGACATTTGT13.125No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCG13.125No Hit
CTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG13.125No Hit
TAACAGCTGTGGTGTCCCCAAGTGCCATGAACCAGGAGCAATTCAGAGAGGAGCTGGCCTATGACCGGATGCCCA13.125No Hit
GCAGAATTCAATGATTCTGACTCCGGCATTTCACTAAACACAAGTCCCAGTGTGGCATCACCAGAACACTCAGTG13.125No Hit
GGGGTCATCTACCCAATGCCAGGGACACCCAAGGGCCAGCAATACTGGGAGTCCCTTGCTGAGCTGTACTAGTTC13.125No Hit
AAATCTCACCACAATTCACACAGAGATAGTCTTGGAACTTGTAACCCCCATGTACTCTGCGTTGATACCTGTCTC13.125No Hit
CTGTTACCCCACCATTTGTCAACCCGGAGCCTCTTTTTTTTCTTTCCAAGAAGGCTGAGTTCTACATTGATGCTG13.125No Hit
GCACAGCTGTGGTGTCCCCAAGTGCCATGACCCAGGAGCCATTCAGAGAGGAGCTGGCCTATGACCGGATGCCCA13.125No Hit
TTTTTTTTTGCCCCGATGCTGGAGGTGGCCCAGATGTCCGAAAGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.125No Hit
TCACGGAACAGAGACTGTGTAATTCCCAGTACGACAGATGTGCTCACAGTAGCTTAATCTCAAATAACCCACACT13.125No Hit
CACAATAACCAAGCAAAATGTGGCAGCAAAGGCAGTGGCTCTGTCTGCAAATACCTGTCTCTTATACACATCTGA13.125No Hit
GTCCTCCCCCTTCCCATGCTGCCAACTTCTAACCGCAATAGTGACTCTGTGCTTGTCTGTTTAGTTCTGTGTATA13.125No Hit
GGACAAGGCCATTAAGAAATTCGTCATTCGAAACATAGTGGAGGCCGCAGCAGTCAGGGACATTTCTGAAGCGAG13.125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)