FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471895
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG200074.239714343233135RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG85671.8154462327424532RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG36380.7709342120598862RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG12680.26870384301592515RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG10400.2203880100446074RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG8550.18118437364244164Illumina PCR Primer Index 10 (96% over 29bp)
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA5360.11358458979222072No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG5320.11273694359974146No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG5250.11125356276290277Illumina PCR Primer Index 10 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)