FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506585
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG745404.947613310898489No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG169041.12200771944497No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA167791.1137108095460928No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA117160.7776527710019681No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA112690.747983021203583No Hit
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG59200.39294165281082716No Hit
GGGCTCCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGC37120.24638503635705916No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCCTGTCTCTTATACACAT36310.2410086387425867No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCTG35470.23543311529054117No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT34810.231052346863934Clontech Universal Primer Mix Short (95% over 21bp)
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTG34660.23005671767606872No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTGTCTC31750.21074151143148245No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG30530.20264372737017824No Hit
GCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT27760.18425777503426624No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCCTGTCTCTTATACACA27730.18405864919669318No Hit
CAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTC26500.17589448985619796No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT26190.1738368562012764Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCTG26070.17304035285098418No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCT25500.16925696193709616No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA25410.16865958442437698Clontech Universal Primer Mix Short (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTC25150.1669338271654105No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCT24690.16388056432262368No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAA24450.16228755762203925No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC23770.15777403863705003Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATCTT23580.15651290833242065No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGA22810.15140201183471227Clontech Universal Primer Mix Short (95% over 22bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCCTGTCTCTT22580.14987538041331885No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCCT22090.14662299173295898No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGCTGTCTCTTATACAC22000.14602561422023982No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA21720.14416710640289132Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTGTCT21580.14323785249421706No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATCTT21280.14124659411848653No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC20960.13912258518437393No Hit
GAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTG20900.1387243335092278No Hit
ATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGG20700.13739682792540747No Hit
GGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCT20540.13633482345835118No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACCTGTCTCTTA20190.13401168868666552No Hit
GTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGGGCTGTCTCTTA20120.13354706173232841No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG19880.13195405503174398No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCCTGTCT19790.1313566775190248No Hit
CAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCT19740.13102480112306975No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC19580.12996279665601343No Hit
GCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA19280.1279715382802829No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC19000.1261130304629344No Hit
CTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC18450.1224623901074284No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTCTG18300.12146676091956313No Hit
GCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCT18160.12053750701088886No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA17840.11841349807677629No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT17740.11774974528486612No Hit
ACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGGGCTG17560.11655499025942778No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATCT17460.11589123746751759No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTCTGTCTCTTATACACATCTGACGCTGCC17450.11582486218832658No Hit
TTTTTGGGGGCTCCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGT17440.11575848690913557No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCT17130.113700853254214No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCG17100.11350172741664095Clontech Universal Primer Mix Short (95% over 22bp)
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCCTGTCTCT16990.11277159934553974No Hit
ACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCC16980.11270522406634872No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGCTGTCTCTTATACA16560.10991746234032597No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTA16530.10971833650275291No Hit
ATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAT16410.10892183315246069No Hit
GAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTT16050.10653232310158406No Hit
GAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGGGCTGTCTCT15950.10586857030967385No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGATCT15790.10480656584261758No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGC15780.10474019056342657Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC15580.10341268497960619No Hit
CTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA15520.10301443330446008No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGC15450.10254980635012295No Hit
ACACAATGCCATTCATTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCA15360.1019524288374038No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCCTGTCTCT15090.1001602962992463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)