FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242184
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC56000.45081888029470674No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC28170.22677799746253371No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTA21350.17187469811235695No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG19410.15625704404500462TruSeq Adapter, Index 6 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG16720.13460163711656245No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT14780.1189839830492101No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC14540.11705190213366136No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAG13440.1081965312707296No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT13060.1051374031544441No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA12810.10312481886741416No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC12530.10087072446594064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)