FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036507
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTA97460.94027343761306No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC85640.8262365811325924No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC49840.48084576370444193No Hit
GTCTTGCTGTGCTGCTATTTGGAATTCGCGTTTCATTTAGCGAAAACGATTATCAATAAACAATTAATTCAGAAC46740.4509376202958591No Hit
CCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTATTGATAATCGTTTTCGCTAAATGAAACGCGAATTCCAAAT46220.44592077043377426No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC39250.3786756867054443No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC38250.36902789850912726No Hit
GTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTT37260.3594765881947734No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTT36280.3500217557623827No Hit
GACCTGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTAACGGGTGTTGACACAATGTGATTT33410.3223326036389528No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG31690.3057384079412874No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC30710.2962835755088967No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGA27910.269269768559209No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC27900.2691732906772458No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25640.24736928935356925TruSeq Adapter, Index 12 (96% over 29bp)
GGGTAAATTAGAGCCTTTGGCTTGTTCCCCGGATAGTAGATTTCATAGCCAAATTATGGAACGGTCTTGCTGTGC24530.2366602444556573No Hit
ATTCAGAACTGGCACGGACCTGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTAACGGGTGT22550.21755762382694954No Hit
CTACCTCCCCGTGCCGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCT22240.21456680948609128No Hit
CAGCAAGACCGTTCCATAATTTGGCTATGAAATCTACTATCCGGGGAACAAGCCAAAGGCTCTAATTTACCCAGA21240.2049190212897742No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTATTGATA20280.19565714462130984No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAAT19900.19199098510670937No Hit
ATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCG19340.1865882237167718No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAA19190.18514105548732426No Hit
GAACTGGCACGGACCTGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTAACGGGTGTTGACA17830.17202006354033308No Hit
CAATTAATTCAGAACTGGCACGGACCTGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTAAC17690.17066937319284867No Hit
AAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTC17060.16459126662916893No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC16440.15860963794745236No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA16370.15793429277371016No Hit
GAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTC16350.15774133700978382No Hit
GTTTATGTACTCGTTCTGGGTAAATTAGAGCCTTTGGCTTGTTCCCCGGATAGTAGATTTCATAGCCAAATTATG15930.15368926596733065No Hit
TAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTATTGATAATCGTTTTCGCTAAAT14190.136902114505739No Hit
ATTAGAGCCTTTGGCTTGTTCCCCGGATAGTAGATTTCATAGCCAAATTATGGAACGGTCTTGCTGTGCTGCTAT13750.1326570876993595No Hit
GTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTATTGATAATCGTTTTCGCT13650.13169230887972777No Hit
GGATAGTAGATTTCATAGCCAAATTATGGAACGGTCTTGCTGTGCTGCTATTTGGAATTCGCGTTTCATTTAGCG12800.12349168891285828No Hit
GTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTATTGATAATCGTTTTCGCTAAATGAAACGCGAA12790.12339521103089512No Hit
GGCACGGACCTGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTAACGGGTGTTGACACAATG12370.11934313998844194No Hit
GTAGATTTCATAGCCAAATTATGGAACGGTCTTGCTGTGCTGCTATTTGGAATTCGCGTTTCATTTAGCGAAAAC12270.11837836116881024No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG12100.11673823717543634No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATTGTTTAT11640.1123002546051305No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA11500.1109495642576461No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGT11060.10670453745126661No Hit
GTTTATTGATAATCGTTTTCGCTAAATGAAACGCGAATTCCAAATAGCAGCACAGCAAGACCGTTCCATAATTTG10940.10554680286770857No Hit
GTGCTGCTATTTGGAATTCGCGTTTCATTTAGCGAAAACGATTATCAATAAACAATTAATTCAGAACTGGCACGG10890.1050644134578927No Hit
TAGCAGCACAGCAAGACCGTTCCATAATTTGGCTATGAAATCTACTATCCGGGGAACAAGCCAAAGGCTCTAATT10820.10438906828415051No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGT10730.10352076734648197No Hit
CGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCTGAATTAATT10500.10130177606132906No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGC10430.10062643088758687No Hit
GTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAGGTCCGTGCCAGTTCT10390.10024051935973417No Hit
GTCTAATTAAAACAAAGCATTGTGATGGCCCTAACGGGTGTTGACACAATGTGATTTCTGCCCATGTACTCTGCG10370.10004756359580785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)