FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002297041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007849
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG56550.5610959578270158RNA PCR Primer, Index 47 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGC51600.5119814575397704No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG18890.1874288707931446RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG17060.16927138886876902RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG16100.15974615244942447RNA PCR Primer, Index 47 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG15920.15796017062079734No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGC14530.14416842205528804No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG14010.13900891899480974RNA PCR Primer, Index 47 (96% over 29bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC13550.13444474321054048No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC11620.11529504915914984No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10710.1062659188033128No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA10680.10596825516520829No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10550.10467837940008871No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10110.10031264604122245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)