FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002297069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138793
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41803.011679263363426Illumina PCR Primer Index 7 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC32682.3545856059023147No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC25401.8300634758237087No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC25261.8199765117837354No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC22551.6247217078671117No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG16491.1881002644225573Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG15111.0886716188856786Illumina PCR Primer Index 7 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC12350.8898143278119214No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCA11990.8638764202805617No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11730.8451434870634686No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11550.8321745332977889No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG10780.7766962310779362No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT10720.7723732464893763No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG10500.7565223029979898Illumina PCR Primer Index 7 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9450.6808700726981909No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT9210.6635781343439511No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA9060.6527706728725512No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC8950.6448452011268581No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG8650.6232302781840583Illumina PCR Primer Index 7 (96% over 27bp)
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT8530.6145843090069384No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA8510.6131433141440851No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG7240.5216401403528996No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG7100.5115531763129264No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG6910.49786372511581994No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT6860.4942612379586867No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC6710.4834537764872868No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC6710.4834537764872868No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT6660.4798512893301536No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG6520.4697643252901803RNA PCR Primer, Index 41 (96% over 31bp)
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC6510.46904382785875365No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG6490.46760283299590033No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA5840.4207704999531677No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG5820.41932950509031436No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC5390.3883481155389681No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA4810.346559264516222No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG4630.33359031075054213No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC4260.30693190578775587No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT4170.30044742890491594No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT4080.29396295202207606No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC4030.29036046486494277No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA4000.2881989725706628No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT3920.2824349931192495No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA3650.2629815624707298No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT3580.2579380804507432No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT3510.25289459843075657No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC3210.2312796754879569No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG3070.2211927114479837No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC3030.21831072172227706No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA2980.2147082345651438Illumina PCR Primer Index 7 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2940.21182624483943716No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC2860.20606226538802389Illumina PCR Primer Index 7 (95% over 21bp)
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC2790.2010187833680373No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT2780.20029828593661064No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2710.19525480391662406No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT2650.1909318193280641No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG2640.19021132189663742Illumina PCR Primer Index 7 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC2560.1844473424452242No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT2520.18156535271951754No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG2510.1808448552880909Illumina PCR Primer Index 7 (96% over 27bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA2480.17868336299381093No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2390.172198886110971No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2380.17147838867954435No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2370.1707578912481177TruSeq Adapter, Index 7 (96% over 26bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2370.1707578912481177No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG2340.1685963989538377No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2290.16499391179670445No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG2220.15995042977671783No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2180.15706844005101123No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT2180.15706844005101123No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC2090.15058396316817133No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA2070.14914296830531798No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC2060.14842247087389135No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT2050.14770197344246466No Hit
GCGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGA2020.14554048114818469No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA1990.14337898885390474No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT1960.14121749655962476Illumina PCR Primer Index 7 (96% over 25bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC1960.14121749655962476No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1850.13329202481393154Illumina PCR Primer Index 7 (96% over 27bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1850.13329202481393154No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC1840.13257152738250488No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC1830.13185102995107822No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC1820.13113053251965157No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC1820.13113053251965157No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG1750.12608705049966495No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC1720.123925558205385No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA1710.12320506077395835Illumina PCR Primer Index 7 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA1710.12320506077395835No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC1700.12248456334253167No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT1700.12248456334253167No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG1700.12248456334253167No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGC1690.12176406591110503No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT1670.12032307104825173Illumina PCR Primer Index 7 (95% over 22bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG1660.11960257361682505No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1660.11960257361682505No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG1640.11816157875397175No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG1630.11744108132254508No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG1600.1152795890282651Illumina PCR Primer Index 7 (95% over 23bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA1600.1152795890282651No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1590.11455909159683847No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG1580.1138385941654118Illumina PCR Primer Index 7 (96% over 27bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA1570.11311809673398515No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG1510.1087951121454252No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG1450.10447212755686525No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1440.1037516301254386No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC1430.10303113269401194No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG1420.1023106352625853Illumina PCR Primer Index 7 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTC1410.10159013783115863No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT1410.10159013783115863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)