FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002297078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644938
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGC145570.8849573661742873No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG66960.4070670140759104Illumina PCR Primer Index 10 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGC55900.3398304373781869No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC46100.2802537238485584No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC41210.25052615964857033No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC39730.24152886005429994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG27250.16565973915126284Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG25030.1521637897598572Illumina PCR Primer Index 10 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG19710.11982214527234461No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC19540.11878867167030004No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTC19200.11672172446621089No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAA19170.11653934677173243No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTC18150.11033850515946497No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG17730.10778521743676661Illumina PCR Primer Index 10 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT16940.10298260481550064No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC16870.1025570568617176No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)