FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002297082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888425
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC67690.3584468538596979No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG57240.30310973430239485TruSeq Adapter, Index 4 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG23880.1264545851701815RNA PCR Primer, Index 4 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC23670.12534254736089598No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC21800.11544011544011544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)