FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002297141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507889
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131452.5881639492093744No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC50220.9887987335815503Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGA40770.802734455757065Clontech Universal Primer Mix Short (95% over 22bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT37990.7479980861959995Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCC35720.7033032808349856Clontech Universal Primer Mix Short (95% over 21bp)
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA28940.5698095449990057No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGAC28160.5544518585753974No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT26830.5282650342889883Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG25670.5054253980692632No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGACTGTCTCTTATACACATCTGACGCTGCCG22200.43710338282577493Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGC21060.4146575334374243Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG19940.3926054708804483No Hit
ATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAC19310.3802011856921493No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAA17350.3416100762174412No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTA17300.34062560913900475No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCG16610.3270399634565821Clontech Universal Primer Mix Short (95% over 22bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA16530.32546481613108375Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGC16210.3191642268290906Clontech Universal Primer Mix Short (95% over 21bp)
TCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG15490.30498790089960603Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC14430.2841171988367537No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTA13800.2717129136484547No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA13300.2618682428640904Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAATAG12960.2551738667307226No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGA12660.2492670642601041No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCCTGTCTCTTATACACATCTGACG12530.24670744985616938Clontech Universal Primer Mix Short (95% over 21bp)
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCG12460.24532919594635835No Hit
GTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAATAG11610.22859325561293906No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCCG11120.21894547824426203Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT10830.21323556918933073No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC10800.21264488894226888No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT10420.205162939146152No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC10380.20437536548340288No Hit
GCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACG10040.19768098935003514Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCG10000.196893415687286Clontech Universal Primer Mix Short (95% over 21bp)
ACACAATGCCATTCATTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCA9710.1911835066323547No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC9690.19078971980098014No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACATC9620.18941146589116914Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGACTGTCTCTTATACACATCTGACGCTGCC9600.18901767905979455Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACT9470.18645806465585985No Hit
ATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGA9150.18015747535386667No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGAC9020.177597860949932Clontech Universal Primer Mix Short (95% over 22bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGACTGTCTCTTATACACATCTGACGCTGC8920.1756289267930591Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGAC8900.17523513996168452Clontech Universal Primer Mix Short (95% over 21bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT8710.1714941650636261No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACT8620.16972212432244052No Hit
ACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCCGACGAC8620.16972212432244052Clontech Universal Primer Mix Short (95% over 21bp)
GAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAAT8480.16696561650281855Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC8260.16263396135769823No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATC7990.15731783913414152Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGA7920.15593958522433052Clontech Universal Primer Mix Short (95% over 21bp)
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGAC7900.15554579839295596No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTA7830.15416754448314493No Hit
ATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCCGA7680.15121414324783566Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTT7520.14806384859683908No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC7500.1476700617654645No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT7210.1419601527105332No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAAT7110.13999121855366037No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC7060.1390067514752239No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGA7000.1378253909811002No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAAT6970.13723471073403834Clontech Universal Primer Mix Short (95% over 21bp)
TTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGA6960.13703781731835105No Hit
ACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACATCTGACG6960.13703781731835105Clontech Universal Primer Mix Short (95% over 21bp)
TCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC6790.1336906292516672Clontech Universal Primer Mix Short (95% over 21bp)
ACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGACGACTT6660.13113101484773249Clontech Universal Primer Mix Short (95% over 22bp)
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGACTT6550.12896518727517234No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACG6550.12896518727517234Clontech Universal Primer Mix Short (95% over 21bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCT6540.12876829385948504Clontech Universal Primer Mix Short (95% over 21bp)
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGACG6320.12443663871436475No Hit
TCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC6250.12305838480455376Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGCTGTCTCTTATACACATCTGACGCTGCCGACG6220.1224677045574919No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT6140.1208925572319936No Hit
GAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAAT6110.12030187698493175No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG6050.11912051649080803No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACAACCCCTGTCTCTTATA6010.11833294282805888Clontech Universal Primer Mix Short (95% over 21bp)
GAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACATCTGACGCTGCC6010.11833294282805888Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTG5960.11734847574962247No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA5890.11597022183981145No Hit
GAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAATAG5870.1155764350084369Clontech Universal Primer Mix Short (95% over 22bp)
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGACTT5850.11518264817706231No Hit
ATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGACG5800.11419818109862588Clontech Universal Primer Mix Short (95% over 22bp)
ATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACATCT5780.11380439426725131Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGACG5750.11321371402018944No Hit
GGGTTGTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATA5690.11203235352606573Clontech Universal Primer Mix Short (95% over 21bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCC5660.11144167327900387Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC5640.11104788644762931No Hit
GTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACATCTGACGCTGCCGA5610.11045720620056745Clontech Universal Primer Mix Short (95% over 21bp)
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGACTTA5570.1096696325378183No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATCTGTCTCTTATACACATCTG5540.10907895229075645No Hit
ATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGACT5360.1055348708083853No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAAT5360.1055348708083853No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGACTGTCTCTTATACACATCTGACGCTGCCGA5320.10474729714563616Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTTAATA5270.10376283006719972No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCCTGTCTCTTATACACATCTGAC5270.10376283006719972Clontech Universal Primer Mix Short (95% over 21bp)
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT5250.10336904323582514No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGACG5230.10297525640445059No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGACT5230.10297525640445059No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTCTGTCTC5150.10140010907895229No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTTA5150.10140010907895229No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACAACCCTGTCTCTTATAC5100.10041564200051586Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)