FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002297205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273461
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC71000.5575357235125379No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG60690.47657525436585807TruSeq Adapter, Index 11 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC39910.31339789754063924No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC35270.27696176011672125TruSeq Adapter, Index 11 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC33640.2641619963234053TruSeq Adapter, Index 11 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC28730.22560565262697485TruSeq Adapter, Index 11 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG25050.19670802639421228RNA PCR Primer, Index 11 (96% over 30bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC24670.1937240323810466TruSeq Adapter, Index 11 (95% over 21bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC13570.1065599967333118TruSeq Adapter, Index 11 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13310.10451831661904055No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACA13240.10396863351135213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)