FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002297206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002297206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434026
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC71940.5016645444364328No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45230.3154057178879602TruSeq Adapter, Index 12 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC39990.27886523675302954No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC33430.2331199015917424No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC31320.2184060818980967No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC25060.17475275901552692No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC24840.1732186166778008No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG19390.13521372694776804RNA PCR Primer, Index 12 (96% over 29bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC14980.10446114645062222No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA14440.10069552434893092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)