FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002300216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3851911
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT159470.4140022965224275No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG79850.20729970136900877No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG76440.19844695269438986No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC75780.1967335174670443No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA60160.15618221708653185No Hit
GTTAGGTATTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT59380.15415724818148704No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG56660.14709581815363854No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG50830.1319604736454191No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT48540.12601537262932608No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT48480.12585960579047645No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT45140.11718858509451544No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT44600.11578668354486903No Hit
GTCGGTGACAAGAAGAGGGATGGCGTGACCTGTGGATGCTGAGGAAGGGC42280.10976369910935119No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA38570.10013211624048428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)