FastQCFastQC Report
Fri 31 May 2024
EGAF00002300221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7030698
Total Bases885.8 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA239890.34120367565211873No Hit
GTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCC156680.22285127308839034No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT142820.20313772544347658No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG140350.199624560747738No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG96930.13786682346475415No Hit
GGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG84990.12088415687887605No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA83600.11890711277884501No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA80440.11441253770251546No Hit
ACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCC71570.1017964361433246No Hit
GTCCAAGTGGCGTAGGGGAGCATAGGGCTCTGCCCCATGATGTACAAGTC70570.1003741022584102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)