FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002300223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240057
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT81880.3655264129439563No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC56160.250707906093461No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC48500.21651234767686717No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC47200.2107089239247037No Hit
CCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC39670.17709370788332618No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT39490.17629015690225738No Hit
ACCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAAC36320.16213873129121267No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA35340.15776384261650483No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA34750.15512998106744605No Hit
CAACCTCTGGTCCTGGAACTTCTCTCAGCCCTGTTCCCACCACGAGCACA31510.14066606340820792No Hit
TGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTA30050.13414837211731667No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA29700.13258591187634958No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC29660.13240734499166762No Hit
CCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAAGTACTCCCAGC28360.12660392123950417No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGA28000.12499681927736661No Hit
CCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAACT27310.12191654051660293No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC26580.11865769487115731No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT22660.10115814017232598No Hit
CAATCTCTCCCCTTACAACCAGCACAACCTCTGCTCCTATAACCAGCATG22560.10071172296062109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)