FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002300241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5419267
Sequences flagged as poor quality0
Sequence length126
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN357710.660070817695456No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT109380.20183541427281587No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC105940.19548769233920382No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC95530.17627845241801152No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC93220.17201588332887086No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC92210.17015216264487432No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGTATCC79660.14699404919521403No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG77920.14378328286832887No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC76790.14169813002385748No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG76570.14129217106298694No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC73820.1362176840521052No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC71130.1312539131214609No Hit
CTGACACTTCCTCAGCATCCACAGGTCACGTCACCCCTCTTCCTGTCACC66320.12237817402242776No Hit
CCGACACTTCCTCAGTATCCACAGGTCACGCCACCCCTCTTCCTGTCACC65880.12156625610068668No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACGCTTCCTCGGCATCC60060.11082679631765698No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACCCCTCTTCCTGTCACC58500.10794817823148407No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA57500.10610291022752708No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC56700.10462669582436147No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT56410.10409156810321396No Hit
GGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGTATCCACAGG55360.10215403669905912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)