FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822696
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG126580.44843653018249224No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA114650.40617197176033126No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA93500.3312436054041951No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG83570.2960644716965624No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC80940.2867471381969578No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC68370.24221524386614782No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT62740.22226977329475084No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG57040.20207631285834535No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA54480.19300696922374921No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC53950.19112933167439922No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC52600.18634666999209265No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT48360.1713255695972928No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA46020.16303562268129476No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG45900.16261049719842308No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC45750.16207909034483345No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG42170.1493961801058279No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT41520.14709341707360624No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT40500.14347985046919684No Hit
CCCTTGAGTACCCTTCTCCACGTAATCGTTGATCTGTTTCTTGGCCTCTT38510.13642985287824122No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT37980.13455221532889125No Hit
CCCTCGTATAACAATAAGGAGCTGCCACAGCATCTGGATTCCTGCAGTAG37870.1341625169695922No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA36860.13058437748875545No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA36270.1284941771979696No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA35300.1250577462114234No Hit
GGTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCC34780.12321553578564606No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC34550.12240071194347532No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG33690.11935397931622817No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC33650.11921227082193761No Hit
CTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTT33300.11797232149689517No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG32430.11489016174607539No Hit
CCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAA31230.11063890691735843No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG31180.11046177129949523No Hit
AGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGT31080.11010750006376882No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC31070.11007207294019619No Hit
GTTCGGAAGGAGCCTCTAGGCTTGGAACCGGGGTAACAGTCGGAGGCGCG30800.10911554060373486No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA30210.10702534031294905No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA29560.10472257728072737No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT28830.10213639725992454No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC28340.10040046820486515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)