FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822696
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC96990.3436076715310469No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC86960.3080742665876878No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA72770.25780317823810994No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC61300.2171682675002905No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC54680.19371551169520204No Hit
TGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG52730.18680722259853702No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC52680.1866300869806738No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC51790.18347707298270874No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT48690.17249466467518995No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA45920.16268135144556836No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA45690.16186652760339762No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC43730.15492281138315991No Hit
GATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAG43690.15478110288886937No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC43520.15417884178813446No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC41440.1468100000850251No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT41030.1453574880185468No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT40960.14510949815353832No Hit
GATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAG40140.14220447402058173No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC39730.14075196195410344No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA39480.13986628386478742No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG38520.13646528000181385No Hit
TCATGAACTACTGCAGGAATCCAGATGCTGTGGCAGCTCCTTATTGTTAT37520.13292256764454977No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA37240.1319306081845158No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC35050.12417206812210736No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA34800.12328639003279133No Hit
GAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC34700.12293211879706492No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG34520.1222944305727574No Hit
CATGAACTACTGCAGGAATCCAGATGCTGTGGCAGCTCCTTATTGTTATA34170.12105448124771497No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT31080.11010750006376882No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA30960.10968237458089712No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA30530.10815900826727355No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT30360.10755674716653865No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC30340.10748589291939338No Hit
GAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCC29700.10521855701074434No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC28810.10206554301277926No Hit
GCTTTTTGAGCAGCTTGGAGAGTACAAATTCCAGAATGCGCTATTAGTTC28470.10086102081130947No Hit
AGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTC28370.10050674957558306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)