FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132810
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG73110.23336876478305418No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG67300.21482311407330798No Hit
GCCAGCATTTGGGTAGTATTCTGGGGTCCGACTATGCGAGTGTGGTGTCA65950.21051388370185234No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA63180.2016719813841248No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA62350.19902260271130393No Hit
CCCTCGTATAACAATAAGGAGCTGCCACAGCATCTGGATTCCTGCAGTAG58850.18785052397049296No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA54780.17485899240617847No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG52930.16895375078603553No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA49850.1591223214941219No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT45430.14501358205572634No Hit
CCTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATC43860.14000210673484828No Hit
CTCGTATAACAATAAGGAGCTGCCACAGCATCTGGATTCCTGCAGTAGTT43070.13748040896192235No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA42210.13473526961418025No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT41820.13349038084020415No Hit
GGGTAGTATTCTGGGGTCCGACTATGCGAGTGTGGTGTCATAGATGACCA37880.12091381220054838No Hit
CCCCGAAGTTGACAGTGAAGGCTTCTGAGTGGTACAACTTTTTAACATCC37590.11998812567630976No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT37290.11903051892709739No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA37180.1186793964523862No Hit
CGGGGTAACAGTCGGAGGCGCGACGGCAGTCCCTTCTGCGTCTGAGCATT37120.11848787510254373No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA35360.11286991550716449No Hit
GTCGGAGGCGCGACGGCAGTCCCTTCTGCGTCTGAGCATTGCGTCAGGTT35340.11280607505721701No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT35080.11197614920789963No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT34630.11053973908408106No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC34520.11018861660936985No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA34070.10875220648555131No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG33670.10747539748660148No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC32950.1051771412884918No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT32470.10364497048975202No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT32350.10326192779006706No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG31870.10172975699132727No Hit
GTTCGGAAGGAGCCTCTAGGCTTGGAACCGGGGTAACAGTCGGAGGCGCG31820.10157015586645854No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG31480.1004848682173512No Hit
GTCCATTACCATGGTAGCACTCCTGCACCCCAGGCCTTTGCTCAGTCGGT31370.10013374574263999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)