FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132810
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1876345.9893194927237845No Hit
GTTTCGTCGAGATGCACACAAGAGTGAGGTTGCTCATCGGTTTAAAGATT74390.23745455357969364No Hit
CTGCAACCTGACGCAATGCTCAGACGCAGAAGGGACTGCCGTCGCGCCTC59330.18938269476923272No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC56310.17974278682716155No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT55770.1780190946785793No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC52350.1671023777375583No Hit
CCCGGTGTCAGGTGGGAGTACTGCAACCTGACGCAATGCTCAGACGCAGA48320.15423852707313881No Hit
CATGAACTACTGCAGGAATCCAGATGCTGTGGCAGCTCCTTATTGTTATA47020.15008889782655188No Hit
CCGGCAATGGCCTGTTCCTCAGCGAGGGCCTGAAGCTAGTGGATAAGTTT45320.14466245958101512No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT45090.14392829440661897No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC38820.12391431334808048No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT37950.12113725377536462No Hit
CCAGAATACTACCCAAATGCTGGCTTGATCATGAACTACTGCAGGAATCC36680.11708338520369892No Hit
CAGCTCCTTATTGTTATACGAGGGATCCCGGTGTCAGGTGGGAGTACTGC35880.11452976720579926No Hit
CCCGGTTCCAAGCCTAGAGGCTCCTTCCGAACAAGCACCGACTGAGCAAA35650.11379560203140311No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA32060.10233624126582844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)