FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540132
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC69860.2750250774369206No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC58250.22931879130690846No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC54790.21569745194344234No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT54380.2140833625969044No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA50900.2003832871677535No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA48460.1907774871542109No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT47460.18684068386997213No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC45030.1772742518892719No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC44170.17388860106482656No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC42350.166723619087512No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTG40020.15755086743523564No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC39990.15743276333670847No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT39440.15526752153037718No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT38180.1503071493922363No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC36900.14526804118841066No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA35460.13959904445910687No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC35130.13829989937530807No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC32090.12633201739122218No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA32070.1262532813255374No Hit
GGATGTGTCTGTGCCCAAGGTAGAAGGTGAAATGAAAGTGCCAGATGTTG31460.12385183132215176No Hit
CTTTGCCGGAAAGCAGCTGGAAGATGGGCGCACCCTGTCTGACTACAACA31170.1227101583697225No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG29610.11656874524631003No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC29520.11621443295072856No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG28750.1131830944218647No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG28710.11302562229049515No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC27800.10944313130183787No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG27470.10814398621803907No Hit
CCTGAAGATGCCCAAGGTGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA27460.10810461818519666No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT27440.1080258821195119No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA27310.10751409769256086No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT25810.10160889276620272No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT25710.10121521243777884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)