FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002300290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8059253
Sequences flagged as poor quality0
Sequence length126
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG575690.7143217863988139No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG316180.39231923851999684No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT311820.38690930784776206No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG233560.28980353390072255No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG232970.2890714561262688No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT192080.23833474392725976No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC187530.23268905939545512No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA186490.23139861721675695No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG180420.22386690180839341No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG167430.20774878267253802No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT156340.19398820213238124No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC156020.19359114300047411No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC139280.17281998716258193No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA135690.16836548002649873No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC134040.16631814387760258No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT131710.16342705707340371No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT128730.15972944390751848No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT121460.15070875675450318No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC119810.148661420605607No Hit
TGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCG117940.14634110630352465No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA116170.1441448729801633No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT113300.14058374889087116No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG109430.1357818150143692No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT109410.135756998818625No Hit
GTCGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGCGT109260.13557087735054352No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT105130.13044633292936703No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT102690.1274187570485751No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC101160.1255203180741441No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT99220.12311314708695706No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA94570.11734338157643145No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC94120.11678501717218705No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACA93500.11601571510411698No Hit
GCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGT93350.11582959363603551No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT89960.11162324845739426No Hit
GTCGGTGACAAGAAGAGAGGTGGCGTGACCTGTGGATACTGAGGAAGCGT89850.11148675938080117No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGGGGTGG89430.11096561927017305No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGG88680.1100350119297657No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG88090.1093029341553119No Hit
GTCGGTGACAAGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC87900.10906718029574204No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGG87270.10828547012979987No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACA83410.10349594435117003No Hit
GGCGTGACCTGTGGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGCGTGG83170.10319815000223967No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC81800.10149824059376222No Hit
CTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGTC81480.10110118146185508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)