FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002300294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446808
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG105070.726219373959779No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT76580.5293031279893393No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA61220.4231383846370769No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA54070.3737192495479704No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC49450.3417868853365478No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA49350.3410957086220148No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA41410.2862162774880979No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC37630.2600897976787521No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG35580.24592067503082649No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG34330.23728096609916452No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG34330.23728096609916452No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC32810.22677508003826355No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC32470.22442507920885146No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT32310.22331919646559878No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC31810.21986331289293395No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC31440.21730595904916203No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG31050.2146103698624835No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC30300.20942654450348627No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG29540.20417360147303582No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA29400.20320595407268965No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT28430.19650153994171998No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT28010.19359859774068156No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC27670.1912485969112695No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT26700.1845441827802998No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG25330.17507506179119828No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT25200.17417653206230543No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT24570.1698221187607478No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA23960.16560594080209676No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT23940.16546770545919015No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC23470.16221917490088525No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG23080.15952358571420672No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG22770.15738093789915455No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA22700.15689711419898147No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG22690.15682799652752819No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT22240.1537177013121299No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT21550.14894858198185248No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC21170.14632211046662721No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC20450.1413456381219899No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT20290.1402397553787372No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC20270.1401015200358306No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG20040.1385118135924048No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG19490.13471034166247353No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA19230.13291328220468782No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC18400.12717651547406428No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG17950.12406622025866597No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT17840.12330592587267973No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG17820.12316769052977312No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT17080.11805298284222925No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC17060.11791474749932265No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT17060.11791474749932265No Hit
CATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAG16900.11680886475606991No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC16690.1153573936555507No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG16620.11487356995537763No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG16510.11411327556939137No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG16010.11065739199672658No Hit
CTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTT15920.11003533295364691No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC15770.10899856788184749No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC15590.10775444979568816No Hit
GGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACA15490.10706327308115521No Hit
CTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAAC15060.10409121320866348No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA14670.10139562402198496No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG14550.10056621196454539No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)