FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002300297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111461
Sequences flagged as poor quality0
Sequence length101-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96010.8638179837169276No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT66910.6020004300645727No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT50660.45579646969169413No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA47310.42565596093790065No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC44450.39992406391227403No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC43200.3886776054220526No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC40890.3678941501321234No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA40710.3662746601095315No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC34140.3071632742849277No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT31520.28359069728942354No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC30470.2741436721576376No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC30080.2706347771086885No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT28010.25201064184888183No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT26720.24040429668697327No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT26010.2340163082645275No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG25580.23014752654389134No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT25410.22861800818922123No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT24470.2201606714045747No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG23080.20765460956344844No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT22520.20261619615982926No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA22310.20072679113347208No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC22010.19802764109581894No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG21640.19469868938271337No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC20390.18345223089249196No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA20170.181472854198213No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC19590.17625449745875024No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC19190.17265563074187937No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG19110.1719358573985052No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG19060.17148599905889636No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA19010.1710361407192875No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC18600.16734730233449488No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT17550.15790027720270886No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC17450.15700056052349115No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC17320.15583092884050811No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG17310.15574095717258635No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT17260.1552910988329775No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC17050.15340169380662028No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC16850.15160226044818487No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC16720.15043262876520183No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC16720.15043262876520183No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT16340.14701370538417452No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT16210.14584407370119148No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC16150.14530424369366088No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT16040.14431455534652138No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT15980.14377472533899074No Hit
TGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCA15760.14179534864471177No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC15750.14170537697679003No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT15610.1404457736258852No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA15320.13783659525615385No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC15300.1376566519203103No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC15240.13711682191277969No Hit
CCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAG15050.13540736022226602No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC15030.13522741688642245No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT14820.13333801186006528No Hit
CAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGA14580.13117869182994274No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA14580.13117869182994274No Hit
CCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCAC14460.1300990318148815No Hit
CACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCA14410.12964917347527263No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT14410.12964917347527263No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT14280.1284795417922896No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG14090.12677008010177596No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA14070.1265901367659324No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC13930.12533053341502762No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG13900.1250606184112623No Hit
CTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGT13790.1240709300641228No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG13730.1235311000565922No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC13430.12083195001893904No Hit
CTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCC13430.12083195001893904No Hit
GTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCC13350.12011217667556487No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC13090.1177729133095988No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC12570.11309438657766668No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAG12540.1128244715739014No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA12510.11255455657013606No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG12470.11219466989844898No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG12460.11210469823052721No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC12430.1118347832267619No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA12400.11156486822299658No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT12220.10994537820040468No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA12110.10895568985326522No Hit
CGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCAC11960.10760611483443863No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA11910.10715625649482978No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTG11800.1061665681476903No Hit
GGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGG11730.10553676647223789No Hit
CGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGA11720.10544679480431612No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG11560.10400724811756779No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG11530.10373733311380247No Hit
CAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTT11520.1036473614458807No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA11470.10319750310627185No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT11470.10319750310627185No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC11410.1026576730987412No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA11350.1021178430912106No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT11340.1020278714232888No Hit
CATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTC11300.10166798475160171No Hit
CTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGC11190.10067829640446223No Hit
AGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACA11180.10058832473654046No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG11160.10040838140069692No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC11120.10004849472900984No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)