FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712948
Sequences flagged as poor quality0
Sequence length101-126
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA74340.20021826322372413No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG71050.1913573796347269No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC70940.19106111908919812No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG68980.18578229482341257No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC68010.18316981546738603No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT67180.18093439498748703No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG66480.17904910060684934No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT63070.16986502369545708No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA59720.16084254344526236No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA58550.15769140855191077No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG55700.15001556714502867No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA54790.14756468445019966No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGGGGTGG53010.14277065016800666No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT49320.13283245550435935No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT49190.13248232940509805No Hit
GCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGT49150.13237459829763304No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCT48830.13151274943791297No Hit
GGGGTGGTGTCACCTGTGGATGCTGAGGAAAGGCTGGTGACAGGAAGAGG47910.12903493396621768No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG46440.12507581576687848No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC45780.12329825249370581No Hit
GGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTG44370.11950073095556414No Hit
GTCGGTGACAAGAAGAGAGGTGGCGTGACCTGTGGATACTGAGGAAGCGT44130.11885434431077409No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC43940.11834262155031526No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATACTGAGGAAGCGT43580.11737304158313017No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGG43520.11721144492193265No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT43140.11618799940101504No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG42100.11338699060692474No Hit
CTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGTC41260.11112463735015948No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT40380.10875455298592923No Hit
GTCGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGCGT40060.10789270412620916No Hit
GTCGGTGACCGGAAGAGGGGTGGCATGACCTGTGGACACTGAGGAAGCGT39800.10719245192768657No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAAGGC39000.10503782977838634No Hit
GGGGTGGTGTCACCTGTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGG38160.1027754765216211No Hit
GGCGTGGTGTCACCTGTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGG37820.1018597621081685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)