FastQCFastQC Report
Fri 31 May 2024
EGAF00002300299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712948
Total Bases461.6 Mbp
Sequences flagged as poor quality0
Sequence length101-126
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187960.5062284739780897No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC100060.2694893653237266No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG97620.2629177677683609No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC91380.24611171500381906No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC89810.24188326903581736No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT73840.19887162438041148No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC66330.17864510895385552No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC62590.16857225040587695No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA60300.16240464450350503No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT56830.15305897093091528No Hit
CCACAGGTCAGGCCATCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCC54460.14667590281361334No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC50030.1347446826618633No Hit
CCACAGGTCACGCCACCCCTCTTCATGTCACCAGCCCTTCCTCAGCATCC49810.13415216157080573No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGTATCC49570.13350577492601567No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACGCTTCCTCAGTGTCC48550.13075863168565788No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC47270.12731123624677748No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC46510.12526434520494228No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACCCCTCTTCCTGTCACC45580.12275959695638075No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG45520.12259800029518324No Hit
CCAGCCCTTCCTCAGCATCCTCAGGTCACACCACCCCTCTTCCTGTCACC45220.12179001698919564No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC45170.12165535310486439No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT44540.11995858816229044No Hit
CCGACACTTCCTCAGTATCCACAGGTCACGCCACCCCTCTTCCTGTCACC44530.11993165538542419No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC44460.11974312594736042No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCAGCACTTCCTCAGCATCC43790.11793862989732148No Hit
CTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACT43680.11764236935179269No Hit
CCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCC43400.11688825159953761No Hit
CACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG43280.11656505827714259No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA42780.11521841943382995No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC42140.11349472171438976No Hit
CCAGCCCTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC41240.11107077179642699No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC40120.10805430078740666No Hit
CCACAGGTCACGCCACCTCTCTTCTTGTCACCGACGCTTCCTCAGTATCC39250.10571114920004267No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACGCTTCCTCGGCATCC38550.10382585481940497No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA38500.1036911909350737No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC38250.10301787151341738No Hit
CAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACTA37890.10204829154623225No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG37260.10035152660365834No Hit
CGCCACCCCTCTTCCTGTCACCGACGCTTCCTCAGTGTCCACAGGTCACG37210.10021686271932707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)