FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230037
Sequences flagged as poor quality0
Sequence length126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155240.6961319475865199No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT47970.21510853855788042No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC39050.17510920222399898No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC37710.16910033331285534No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA36340.1629569374857906No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA32040.14367474620376253No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT31580.1416120001596386No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC27560.12358539342620772No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC27340.1225988627094528No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC27250.12219528196168943No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC27200.1219710704351542No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT26710.11977379747510916No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC26120.11712810146199366No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC25940.11632093996646693No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC25860.1159622015240106No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT25750.11546893616563311No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT24660.11058112488716554No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA24380.10932554033856838No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC24300.10896680189611205No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC24170.1083838519271205No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC24080.1079802711793571No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA23730.10641079049361064No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT23600.10582784052461909No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC23550.10560362899808388No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC23280.10439288675479375No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG22950.10291309067966138No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG22850.10246466762659096No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA22770.10210592918413462No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC22670.1016575061310642No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG22460.10071581771961631No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)