FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179635
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC101550.465903694884694No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC98080.4499835981712535No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC94950.4356233956602826No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT57080.26187870904990973No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA52190.2394437600790958No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA47770.21916513544698998No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC39710.18218646700020874No Hit
GTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCC36730.1685144531079745No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG36090.16557818166803157No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC35720.16388064974181457No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT34980.16048558588938056No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC34770.1595221218231493No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT33040.15158501308705355No Hit
AGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA32690.14997923964333476No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG32290.14814406999337043No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA31330.14373966283345607No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA30660.1406657536697658No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG30570.14025284049852385No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC30530.1400693235335274No Hit
GTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCC29290.13438029761863798No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT27500.12616791343504763No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA27460.1259843964700512No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT25700.11790965001020814No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC25290.1160286011189947No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA25070.11501925781151431No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA24960.11451458615777411No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC24110.11061485065159993No Hit
TGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG24070.11043133368660349No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT23200.10643983969793108No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC22510.10327417205174261No Hit
AGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGA22380.1026777419155042No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG22160.10166839860802383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)