FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351854
Sequences flagged as poor quality0
Sequence length126
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC93550.3977712902246483No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC85540.3637130536164235No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG75270.3200453769664274No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA74950.3186847482879464No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC68420.2909194193176957No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC66840.2842013152176963No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT58990.250823392948712No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT58040.2467840265594718No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT58000.2466139479746617No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG56160.23879033307339656No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT53290.22658719461327104No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT52750.22429113371833456No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT52670.22395097654871435No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA52380.22271790680884102No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC51660.21965649228225903No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC51490.21893365829681605No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA49880.21208799525820904No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC49640.2110675237493484No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT49160.20902658073162708No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC46890.19937462104365322No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC45750.19452738137656503No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT45530.19359194916010944No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT44740.19023289711010974No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC42730.18168644822340163No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT42080.1789226712202373No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG41410.17607385492466793No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG41370.17590377633985782No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTG41020.17441558872276935No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT40780.17339511721390868No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA40490.17216204747403538No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC40320.1714392134885924No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA38490.1636581182335298No Hit
ACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT37860.16097938052277055No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC37330.15872583927403655No Hit
CAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGG37140.15791796599618854No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG36730.1561746605018849No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTG36090.15345340314492312No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG36010.1531132459753029No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG36000.15307072632910038No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG35430.15064710649555627No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA35070.14911639923226527No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG34700.14754317232277175No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC34120.14507703284302512No Hit
AGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG33530.14256837371707598No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA33420.14210065760884816No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA32880.13980459671391168No Hit
CATGGATGAAATTGAAAAGTACAAAGAAGTGGAAGAAGACCAAGACCCAT32620.13869908591264596No Hit
CGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCAC32550.13840144838922824No Hit
AGTACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAAAAGGACCAAGAA32060.13631798572530437No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA31910.13568019103226647No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC31110.13227861933606422No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG30760.13079043171897575No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT30760.13079043171897575No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA30630.13023767631834288No Hit
AGAAGAGGAAGAAGACCAAGACCCACCATGCCCCAGGCTCAGCAGGGAGC30630.13023767631834288No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG30550.12989751914872266No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG30410.12930224410188726No Hit
GATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA30210.12845185117783672No Hit
GCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTG30080.12789909577720385No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA29130.12385972938796372No Hit
CTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGG28650.12181878637024238No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC28460.12101091309239434No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC28370.12062823627657158No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA28300.1203305987531539No Hit
GGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGG27480.11684398776454662No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT27380.11641879130252134No Hit
TTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGG26940.1145479268696101No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT26940.1145479268696101No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG26820.11403769111517977No Hit
GAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC26760.11378257323796459No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC26610.11314477854492669No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCT26320.11191170880505337No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA26320.11191170880505337No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC26310.11186918915885086No Hit
CAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCAC26260.11165659092783821No Hit
CTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAA26160.11123139446581293No Hit
CCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCT25860.1099558050797371No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG25830.10982824614112952No Hit
ACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACT25610.1088928139246739No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC25010.10634163515252223No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC24840.10561880116707927No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC24820.1055337618746742No Hit
GGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT24810.10549124222847166No Hit
ACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACT24730.10515108505885144No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC24670.10489596718163627No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC24480.10408809390378825No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT24430.10387549567277561No Hit
CAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCAC24380.10366289744176296No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC24290.10328022062594021No Hit
CAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGA24090.10242982770188966No Hit
CATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTC24090.10242982770188966No Hit
AGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA23910.10166447407024416No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA23810.10123927760821887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)