FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008228
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG173860.5779482140316492No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG137280.456348388486511No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA131150.4359709436917681No Hit
CCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCAC117680.3911937526011991No Hit
GGGCAGCTGAGCAGATGACACCAGCGTCTTCACTGTGCTGACAGTTATGG114650.38112137776790855No Hit
CTGCTGTTGATGCACGAGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC111560.37084954996762215No Hit
CCTCCATTCACCAGTCTCAGGGCCAAAGTGGATTCAGATCCTGCTGTTGA98910.3287982160926632No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG98380.3270363815508665No Hit
GTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT95230.31656510078358424No Hit
GTCGGCAACGTGTCTGGACTGGGCGTCGACCAGGAGTGGGCAGCTGAGCA94310.3135068219563145No Hit
CCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCA86900.28887438053232667No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT85850.28538395360989927No Hit
CTCCACTCGGCCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCA85600.28455289958074986No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA81900.27225329994933894No Hit
GGCAGCTGAGCAGATGACACCAGCGTCTTCACTGTGCTGACAGTTATGGG76670.2548676496595338No Hit
CTGCAAACCACATTGGCATCATTGGTGTCCCAGTAGTCATCACACACGGT76370.25387038482455454No Hit
CCCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCT74370.24722195259135943No Hit
CTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCACC71180.23661770317941325No Hit
CCCAGCTGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC71160.23655121885708133No Hit
CGCACATCATCCAGGACAATGGGTCCTGAGCCCTGGCCAAACTGGGCATT71020.23608582860075766No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT71000.23601934427842572No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA70650.23485586863761657No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG70610.23472289999295265No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC69320.23043466120254183No Hit
GTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATG68790.22867282666074515No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT68760.2285731001772472No Hit
CCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTC66340.22052849717508113No Hit
CCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC66200.22006310691875747No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT65280.21700482809148777No Hit
CCCAGCTATCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC62780.20869428779999388No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT62030.2062011257125457No Hit
CTCCATTCACCAGTCTCAGGGCCAAAGTGGATTCAGATCCTGCTGTTGAT61580.20470522846007685No Hit
GTCCTGAGCAGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCA61150.20327581552993987No Hit
CGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCATT57240.1902781305140435No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT56550.18798442139359117No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG56290.1871201252032758No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC55430.18426129934300192No Hit
CTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCG54250.1803387243254168No Hit
GTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAG53720.17857688978362013No Hit
CCAGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGG50280.16714158634252457No Hit
CCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCC50140.16667619608620093No Hit
GTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCACTCGGC48980.16282010539094777No Hit
GTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGC48650.1617231140724706No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTACTGTCGA48160.1600942481753378No Hit
CTGCAGACGACATTGGCATCATTGGTGTCCCAGCTGTCATCGCACACGGT45120.14998863118088124No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT43590.144902580522487No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG43480.14453691674966127No Hit
GCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCG43000.14294129301369443No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC42460.14114621631073176No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT42370.140847036860238No Hit
CTCGTGTCCTGAGCAGCGCACATCATCCAGGACAATGGGTCCTGAGCCCT42120.1400159828310886No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA42110.13998274066992264No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTTAGATCCTGCTGTTGA41770.13885250719027944No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC41740.13875278070678154No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC41650.13845360125628775No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC41430.13772227371063628No Hit
ATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCT41010.13632610294166533No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT40470.13453102623870267No Hit
GCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATG40460.13449778407753665No Hit
AGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCA39860.13250325440757815No Hit
GGCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTC39770.1322040749570844No Hit
CAGCTATCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCAC39330.13074141986578147No Hit
CCCAGCTGTCATCGCACACGGTGCCCCAGGAGCCTTGGTATAGGACCTCC39090.12994360799779803No Hit
GCCCCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCAC39010.12967767070847022No Hit
CTGGGGCTGACGTGGCCCAGCCACAGCCCAGCTGCCTGCAAACCACATTG38750.1288133745181549No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA38540.1281152891336694No Hit
CAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCAC38470.12788259400550755No Hit
CATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGG38350.12748368807151583No Hit
GCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCAT37610.1250237681452337No Hit
CAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCC37420.12439216708308014No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT37320.12405974547142037No Hit
CGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCCAGCTGCCTGCA36970.12289626983061125No Hit
CTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCCA36850.12249736389661954No Hit
CGCACATCATCCAGGACAATGGGTCCTGAGCCCTGGCCAAACCGGGCATT36650.12183252067330003No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC36590.12163306770630418No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT35840.11913990561885603No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG34770.11558299437409664No Hit
GCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTT34700.11535029924593483No Hit
AGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGC34240.11382115983229996No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA33980.1129568636419846No Hit
GCATCATTGGTGTCCCAGCTGTCATCACACACGGTGCCCCAAGAGCCTCG33950.11285713715848665No Hit
CTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATGGCCACAGTTGT33790.11232526257983104No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC33510.11139448206718373No Hit
GGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGAT32600.10836944540107997No Hit
GGCAGCTCCACAGGTAGGACTCATAGCCTGAGCAGCGCACGTCATCCAGG32390.1076713600165945No Hit
GTCCCAGCTGTCATCGCACACGGTGCCCCAGGAGCCTTGGTATAGGACCT32060.1065743686981173No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA31950.10620870492529158No Hit
CATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCACTCGGCCC31670.10527792441264426No Hit
CTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGAT31650.1052114400903123No Hit
GTCCCAGCTGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT31280.10398148012717122No Hit
CCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCA31230.10381526932134134No Hit
GCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCA30450.10122238075039525No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)