FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008228
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300861.0001236608395374No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC114430.3803900502222571No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC91200.3031685098336961No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC82830.2753448209377747No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT77540.25775971768097367No Hit
GTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCC76830.2553995242381894No Hit
CGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTC72300.24034082523000252No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA70020.23276161248416014No Hit
CTCACATGCATCAACAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGG67400.22405216625867455No Hit
GTAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAG66690.2216919728158903No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT66490.2210271295925708No Hit
GTCCTACCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCATAACTGTC65850.21889963127794837No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC60830.20221206637262867No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC60030.19955269347935062No Hit
CAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGAC56760.18868250677807666No Hit
CAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGAT56400.18748578897610155No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC55370.1840618463760061No Hit
TCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCA52770.17541888447285245No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG52440.17432189315437527No Hit
AGTGGAGGTCCTATACCGAGGCTCCTGGGGCACCGTGTGTGATGACTACT51770.17209466835625492No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC51360.1707317397484499No Hit
CATGCATCAACAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAA50720.1686042414338275No Hit
GGCAGCTGGGCTGTGGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTT50070.1664435009580391No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC49660.1650805723502341No Hit
GGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAG49440.16434924480458263No Hit
CAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCTGGGCCACGT48740.16202229352296435No Hit
CATCTGCTCAGCTTCCCAGTCCCGGCCAACACCTAGTCCAGACACTTGGC48070.159795068724844No Hit
CACGAGTCTTACCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAA47460.1577672968937195No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG45770.15214937165666964No Hit
CTGGGCTGTGGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCA44910.14929054579639575No Hit
GTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTG44800.14892488202357002No Hit
CATCTGCTCAGCTGCCCACTCCTGGTCGACGCCCAGTCCAGACACGTTGC44750.14875867121774014No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT42900.14260887140203468No Hit
CTCAGGCCAGAAAGTTGGCCTGTCAGGATATCACCACCTGTACCCACAGA41050.13645907158632922No Hit
GGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGG40970.13619313429700142No Hit
CACCTAGTCCAGACACTTGGCCAACCTCACATGCATCAACAGCAGGATCT40680.13522911162318813No Hit
GGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGT39780.13223731711825035No Hit
GTGGAGGTCCTATACCGAGGCTCCTGGGGCACCGTGTGTGATGACTACTG37670.12522322111222953No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37500.12465810437240793No Hit
GCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCAT37260.12386029250442453No Hit
GGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAGGCTCCTG36950.12282978550827929No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC36420.12106795096648258No Hit
CAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAGG36090.11997095964800542No Hit
CGACAGTAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGT36050.1198379910033415No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG35720.1187409996848643No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC34950.11618135327508419No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT34910.1160483846304203No Hit
CTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCT34570.11491815115077715No Hit
ACCTCACATGCATCAACAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCT34260.11388764415463189No Hit
GCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAG33990.11299010580315055No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA33740.11215905177400116No Hit
CATCTGCTCAGCTCCCCAGTCCCGGCCGACACCCAGCCCAGATACTTGGC33690.11199284096817129No Hit
CTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGAC33660.11189311448467337No Hit
CCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGA33540.11149420855068165No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC33210.11039721723220448No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC32430.10780432866125839No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA32430.10780432866125839No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG31960.10624194708645754No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT31690.10534440873497622No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG31510.10474604983398865No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT31490.1046795655116567No Hit
GCCGAGTGGAGGTCCTATACCGAGGCTCCTGGGGCACCGTGTGTGATGAC31100.10338312122618366No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC30940.10285124664752804No Hit
GCCAGACACATTGCCGACCATCACCTTGCCTGCATCGACAGTAGGATCTG30910.10275152016403011No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA30840.1025188250358683No Hit
CAGACACATTGCCGACCATCACCTTGCCTGCATCGACAGTAGGATCTGAA30330.10082347481640355No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC30170.10029160023774794No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)