FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110149
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT138890.2717924663253459No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG130280.2549436425434953No Hit
GTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGCCAC86640.1695449584738136No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC83840.16406566618703292No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA83060.162539291907144No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC79940.15643379478758837No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG71310.13954583320368938No Hit
GTGGAGGTGTGGGCAGTCTCTGGAGTGGAGGAGGGTGTGGCTGTGGACAT64280.12578889578366503No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT63990.12522139765396273No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC62850.12299054293720202No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT60330.11805917987909942No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG57270.11207109616568911No Hit
GGGGAGTGTGTGGTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGT57080.111699287046229No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA57070.11167971814520478No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG55430.10847041837723323No Hit
GTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAG55340.10829429826801527No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT54970.10757024893011927No Hit
GTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAG53500.1046936204795594No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG51430.10064285796754653No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)