FastQCFastQC Report
Fri 31 May 2024
EGAF00002300319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110149
Total Bases643.8 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN854181.6715363876865432No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA136410.2669393788713401No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC79510.15559233204354708No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT75470.1476864960297635No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC69470.1359451554152335No Hit
GTTCAAGATGTGCTTCAACTATGAAATCCGTGTGTTCTGCTGCAACTACG63270.12381243678021912No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA62640.12257959601569346No Hit
CCTGGATCCTCACAGAGCCGAGCACTACAGCCACCGTGACGGTGCCCACC61250.11985951877332736No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT58080.11365617714865066No Hit
GGGAAGTTCAAGATGTGCTTCAACTATGAAATCCGTGTGTTCTGCTGCAA57290.11211023396773753No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT56920.11138618462984151No Hit
GCCAAGCCCTGAGCTCATTCAACACAGCCAAAACCTCTACATCCCTACAT56060.1097032591417589No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC55840.10927274331922611No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC52040.10183656093002377No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC51460.10070156467061918No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)