FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807185
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN329571.174023087185205No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT93650.3336082231844356No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC89650.31935907323528734No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC81400.28997020146516883No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG77990.27782280113351987No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA72940.2598332493227201No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC71870.2560216017113229No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG70200.25007258160755347No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA69920.24907514111111312No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA65550.23350794479166853No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT65360.23283111016908398No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC61500.21908068046815585No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC57440.20461779326977025No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC57160.20362035277332988No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC53610.1909742321934607No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC52490.18698447020769918No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC49310.17565639599812624No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG47660.16977862164410254No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC46800.16671505440503565No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT46540.165788859658341No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC46150.16439956753829904No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA45770.16304589829312996No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG45390.16169222904796085No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT45310.16140724604897788No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT45200.1610153944253763No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC45140.1608016571761391No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA44910.15998233105406306No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC44590.1588423990581312No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA44470.15841492455965672No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT44040.1568831409401233No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG42060.14982981171529486No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC41520.14790617647215984No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG40670.1448782321079658No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT40560.1444863804843642No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC39830.14188591061864464No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT39680.14135156749555158No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39110.13932106362779795No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC38640.13764678850877302No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG37910.13504631864305344No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG37640.13408450102148595No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC36650.1305578364090717No Hit
CAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCAC36630.13048659065932597No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA36610.13041534490958023No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC36550.13020160766034303No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAG35410.12614059992483573No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG35310.12578437117610702No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG35300.12574874830123414No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT35170.12528565092788682No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC34950.12450194768068368No Hit
GACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA33400.1189804020753887No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC33260.1184816818271685No Hit
GGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCT32640.11627306358505052No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC32590.11609494921068614No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC32520.11584558908657605No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA32340.11520437733886439No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT32320.11513313158911863No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC32300.1150618858393729No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG32030.11410006821780537No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT31930.11374383946907668No Hit
GGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGAC31870.11353010221983945No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC31850.1134588564700937No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT31690.11288889047212777No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT31660.11278202184750916No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG31450.11203394147517887No Hit
CATGAAATCTAGCTGGGGCTGTGTGGTTTCTGATTCCCCCTGGCTTATTC31410.11189144997568738No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT30820.109789700358188No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA30740.10950471735920506No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG30360.10815104811403595No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC30360.10815104811403595No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT30040.10701111611810409No Hit
CAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCAC29980.10679737886886685No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCT29720.10587118412217222No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC29280.10430377762776591No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC29280.10430377762776591No Hit
CAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGG29100.10366256588005421No Hit
CAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGA29020.10337758288107125No Hit
ATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTAC28760.10245138813437661No Hit
GGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGAC28700.10223765088513939No Hit
TCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG28180.1003852613917501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)