FastQCFastQC Report
Fri 31 May 2024
EGAF00002300335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324309
Total Bases544.8 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC84520.19545319263725142No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC78660.18190189461483905No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC77300.1787568834697058No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG63950.14788489906711108No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA60860.14073924874471272No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT60460.13981424546673238No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC57750.13354734825841538No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC57680.13338547268476883No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT55220.12769670252518958No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG49010.1133360266345444No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC47690.11028351581720916No Hit
CCACAGGTCACGCCACCCCTCTTCATGTCACCAGCCCTTCCTCAGCATCC44530.10297598992116427No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC43510.10061723156231436No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)